- 专利标题： Serum-free stable transfection and production of recombinant human proteins in human cell lines
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申请号： US14506107申请日： 2014-10-03
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公开(公告)号： US09273325B2公开(公告)日： 2016-03-01
- 发明人: Carola Schroeder , Haiyan Ding , Cathleen Wegmann
- 申请人： Carola Schroeder , Haiyan Ding , Cathleen Wegmann
- 代理机构： Thompson Hine LLP
- 优先权： EP05105965 20050630
- 主分类号： C12N15/85
- IPC分类号： C12N15/85 ; C12N5/071 ; C12P21/02 ; C07K14/535 ; C07K14/755 ; C07K14/81 ; C12N9/64
摘要：
The present invention relates to an improved method for the serum-free production of an immortalized human cell line stably transfected under serum-free conditions with a specific vector carrying the gene coding for the protein of interest. Furthermore the invention relates to a production cell line obtained by said method, a production method for said protein of interest utilizing said production cell line, and the specific vector carrying the gene of interest itself.
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