发明授权
US09284567B2 Method for gene introduction into hordeum plant using agrobacterium, and method for production of transformed plant of hordeum plant
有权
使用农杆菌将基因引入大麦植物的方法，以及用于生产大麦植物转化植物的方法
- 专利标题： Method for gene introduction into hordeum plant using agrobacterium, and method for production of transformed plant of hordeum plant
- 专利标题（中）： 使用农杆菌将基因引入大麦植物的方法，以及用于生产大麦植物转化植物的方法
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申请号： US13812412申请日： 2011-07-29
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公开(公告)号： US09284567B2公开(公告)日： 2016-03-15
- 发明人: Yukoh Hiei , Yuji Ishida
- 申请人： Yukoh Hiei , Yuji Ishida
- 申请人地址： JP Tokyo
- 专利权人： JAPAN TOBACCO INC.
- 当前专利权人： JAPAN TOBACCO INC.
- 当前专利权人地址： JP Tokyo
- 代理机构： Birch, Stewart, Kolasch & Birch, LLP
- 优先权： JP2010-170871 20100729
- 国际申请： PCT/JP2011/067493 WO 20110729
- 国际公布： WO2012/015039 WO 20120202
- 主分类号： A01H4/00
- IPC分类号： A01H4/00 ; C12N15/90 ; C12N15/82
摘要：
An object of the present invention is to provide a method of gene introduction, which can transform a Hordeum plant at a higher efficiency compared to that in known Agrobacterium methods, and a method of producing a transformed Hordeum plant. The method of the invention includes a step of subjecting an immature embryo tissue of a Hordeum plant to centrifugation treatment and/or pressurization treatment before the inoculation with Agrobacterium, during the coculture step, and/or after the coculture step, and is characterized in that the coculture medium satisfies at least one of a) containing an antiauxin; b) containing a cytokinin; and c) containing a phenoxy auxin in an amount of less than 2 μM and/or a benzoic auxin in an amount of less than 5 μM, or not containing any phenoxy auxin and/or benzoic auxin.
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