发明授权
- 专利标题： Method for increasing yield of total flavonoids in Ganoderma lucidum mycelium
- 专利标题（中）： 增加灵芝菌丝体总黄酮产量的方法
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申请号： US14373381申请日： 2013-04-07
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公开(公告)号： US09284585B2公开(公告)日： 2016-03-15
- 发明人: Qiang Yao , Zhiyuan Gong , Jianchang Su , Zhaohui Liu , Jilei Wang , Xingcheng Du , Xiao Liu , Xiaoyan Zhu , Qingxin Zhou , Shan Zhu , Yanwen Yang
- 申请人： SHANDONG QIHE BIO-TECHNOLOGY CO., LTD , INSTITUTE OF AGRICULTURAL RESOURCES AND ENVIRONMENT, SHANDONG ACADEMY OF AGRICULTURAL SCIENCES
- 申请人地址： CN Jinan CN Jinan
- 专利权人： SHANDONG QIHE BIO-TECHNOLOGY CO., LTD,INSTITUTE OF AGRICULTURAL RESOURCES AND ENVIRONMENT, SHANDONG ACADEMY OF AGRICULTURAL SCIENCES
- 当前专利权人： SHANDONG QIHE BIO-TECHNOLOGY CO., LTD,INSTITUTE OF AGRICULTURAL RESOURCES AND ENVIRONMENT, SHANDONG ACADEMY OF AGRICULTURAL SCIENCES
- 当前专利权人地址： CN Jinan CN Jinan
- 代理机构： CBM Patent Consulting, LLC
- 优先权： CN201210110969 20120416
- 国际申请： PCT/CN2013/000394 WO 20130407
- 国际公布： WO2013/155868 WO 20131024
- 主分类号： C12P17/06
- IPC分类号： C12P17/06 ; C12N1/14 ; C12N1/38
摘要：
A method for increasing the yield of total flavonoids in Ganoderma lucidum mycelium by an expansin comprises: (1) inoculating Ganoderma lucidum into the PD liquid fermentation medium, activating the culturing of the strains and obtaining a seed solution; (2) inoculating the seed solution into a liquid fermentation medium, culturing and adding the expansin solution, then further culturing, isolating and obtaining Ganoderma lucidum mycelium; (3) extracting flavonoids from the Ganoderma lucidum mycelium and obtaining total flavonoids. A plant expansin is used for the liquid fermentation production of flavonoids ingredients of Ganoderma lucidum, and the fermentation process and extraction method are optimized to increase greatly the yield of total flavonoids in Ganoderma lucidum.
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