A display apparatus includes: a display panel including first to third sub pixels which receive a first grayscale data, a second grayscale data and a third grayscale data, respectively; a light source part which provides light to the display panel and sequentially turns on first and second light sources, which emit a first light and a second light having a color different from the first light, respectively; and a color conversion layer including a first photoluminescence part which is excited by the first light to emit light having a first primary color, a second photoluminescence part which is excited by the first light to emit light having a second primary color, and a third photoluminescence part which is excited by the first light to emit light having a third primary color, where the first to third photoluminescence parts overlap the first to third sub pixels, respectively.