发明授权
US09297048B2 Antibiotic resistance profile for Neisseria gonorrhoeae and use of same in diagnosis and treatment of gonorrhea
有权
淋病奈瑟氏球菌的抗生素耐药性及其在淋病诊断和治疗中的应用
- 专利标题: Antibiotic resistance profile for Neisseria gonorrhoeae and use of same in diagnosis and treatment of gonorrhea
- 专利标题(中): 淋病奈瑟氏球菌的抗生素耐药性及其在淋病诊断和治疗中的应用
-
申请号: US13134252申请日: 2011-06-02
-
公开(公告)号: US09297048B2公开(公告)日: 2016-03-29
- 发明人: Sergey Balashov , Eli Mordechai , Martin E. Adelson , Scott E. Gygax
- 申请人: Sergey Balashov , Eli Mordechai , Martin E. Adelson , Scott E. Gygax
- 申请人地址: US NJ Hamilton
- 专利权人: Medical Diagnostic Laboratories, LLC
- 当前专利权人: Medical Diagnostic Laboratories, LLC
- 当前专利权人地址: US NJ Hamilton
- 代理商 Siu K. Lo
- 主分类号: C12Q1/68
- IPC分类号: C12Q1/68
摘要:
The present invention relates to an antibiotic resistance profile for Neisseria gonorrhoeae by assessing the presence of mutations (e.g., SNP) in antibiotic resistant genes that confer bacterial resistance against antibiotics such as penicillin, tetracycline, fluoroquinolones, cephalosporin, macrolides and spectinomycin. There is provided a method and a kit for generating an antibiotic resistance profile for Neisseria gonorrhoeae by utilizing a multiplex PCR to amplify segments of antibiotic-resistant genes, allele-specific primer extension to detect gene mutation, and detection of such gene mutations with gel electrophoresis, capillary electrophoresis, or DNA microarray. The present method provides useful information to physicians relating the antibiotic susceptibility of Neisseria gonorrhoeae against different classes of antibiotics. Relying on this personalized diagnostic tool, physicians can better inform about antibiotic susceptibility and thereby open up medical intervention avenues for treating Neisseria gonorrhoeae with antibiotics. There is provided a therapeutic application of the antibiotic resistance profile that has advantages of: (i) providing a more effective regime for gonorrhea treatment; and (ii) halting the evolutionary pressures towards antibiotic resistance in the Neisseria gonorrhoeae therapy.