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    ANTIBIOTIC RESISTANCE PROFILE FOR NEISSERIA GONORRHOEAE AND USE OF SAME IN DIAGNOSIS AND TREATMENT OF GONORRHEA
    1.
    发明申请
    ANTIBIOTIC RESISTANCE PROFILE FOR NEISSERIA GONORRHOEAE AND USE OF SAME IN DIAGNOSIS AND TREATMENT OF GONORRHEA 审中-公开

    公开(公告)号：US20170260570A1

    公开(公告)日：2017-09-14

    申请号：US15067700

    申请日：2016-03-11

    申请人： Sergey Balashov ,  Eli Mordechai ,  Martin E. Adelson ,  Scott E. Gygax

    发明人： Sergey Balashov ,  Eli Mordechai ,  Martin E. Adelson ,  Scott E. Gygax

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/689 ,  C12Q2600/156 ,  C12Q2600/16

    摘要： The present invention relates to an antibiotic resistance profile for Neisseria gonorrhoeae by assessing the presence of mutations (e.g., SNP) in antibiotic resistant genes that confer bacterial resistance against antibiotics such as penicillin, tetracycline, fluoroquinolones, cephalosporin, macrolides and spectinomycin. There is provided a method and a kit for generating an antibiotic resistance profile for Neisseria gonorrhoeae by utilizing a multiplex PCR to amplify segments of antibiotic-resistant genes, allele-specific primer extension to detect gene mutation, and detection of such gene mutations with gel electrophoresis, capillary electrophoresis, or DNA microarray. The present method provides useful information to physicians relating the antibiotic susceptibility of Neisseria gonorrhoeae against different classes of antibiotics. Relying on this personalized diagnostic tool, physicians can better inform about antibiotic susceptibility and thereby open up medical intervention avenues for treating Neisseria gonorrhoeae with antibiotics. There is provided a therapeutic application of the antibiotic resistance profile that has advantages of: (i) providing a more effective regime for gonorrhea treatment; and (ii) halting the evolutionary pressures towards antibiotic resistance in the Neisseria gonorrhoeae therapy.

    Antibiotic resistance profile for Neisseria gonorrhoeae and use of same in diagnosis and treatment of gonorrhea
    2.
    发明授权
    Antibiotic resistance profile for Neisseria gonorrhoeae and use of same in diagnosis and treatment of gonorrhea 有权
    淋病奈瑟氏球菌的抗生素耐药性及其在淋病诊断和治疗中的应用

    公开(公告)号：US09297048B2

    公开(公告)日：2016-03-29

    申请号：US13134252

    申请日：2011-06-02

    申请人： Sergey Balashov ,  Eli Mordechai ,  Martin E. Adelson ,  Scott E. Gygax

    发明人： Sergey Balashov ,  Eli Mordechai ,  Martin E. Adelson ,  Scott E. Gygax

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/689 ,  C12Q2600/16

    摘要： The present invention relates to an antibiotic resistance profile for Neisseria gonorrhoeae by assessing the presence of mutations (e.g., SNP) in antibiotic resistant genes that confer bacterial resistance against antibiotics such as penicillin, tetracycline, fluoroquinolones, cephalosporin, macrolides and spectinomycin. There is provided a method and a kit for generating an antibiotic resistance profile for Neisseria gonorrhoeae by utilizing a multiplex PCR to amplify segments of antibiotic-resistant genes, allele-specific primer extension to detect gene mutation, and detection of such gene mutations with gel electrophoresis, capillary electrophoresis, or DNA microarray. The present method provides useful information to physicians relating the antibiotic susceptibility of Neisseria gonorrhoeae against different classes of antibiotics. Relying on this personalized diagnostic tool, physicians can better inform about antibiotic susceptibility and thereby open up medical intervention avenues for treating Neisseria gonorrhoeae with antibiotics. There is provided a therapeutic application of the antibiotic resistance profile that has advantages of: (i) providing a more effective regime for gonorrhea treatment; and (ii) halting the evolutionary pressures towards antibiotic resistance in the Neisseria gonorrhoeae therapy.

    摘要翻译： 本发明涉及通过评估抗生素抗性基因中的突变（例如SNP）的存在，其对抗生素如青霉素，四环素，氟喹诺酮，头孢菌素，大环内酯和壮观霉素提供细菌耐药性，涉及淋病奈瑟氏球菌抗生素抗性谱。 提供了一种通过利用多重PCR扩增抗生素抗性基因片段，等位基因特异性引物延伸以检测基因突变以及用凝胶电泳检测这种基因突变来产生淋病奈瑟氏球菌抗生素抗性谱的方法和试剂盒 ，毛细管电泳或DNA微阵列。 本方法为医生提供了与淋病奈瑟氏球菌对不同类型抗生素的抗生素敏感性有关的有用信息。 依靠这种个性化诊断工具，医生可以更好地了解抗生素敏感性，从而开辟用抗生素治疗淋病奈瑟氏球菌的医疗干预途径。 提供了抗生素抗性谱的治疗应用，其优点在于：（i）为淋病治疗提供更有效的方案; 和（ii）阻止淋病奈瑟氏球菌治疗中抗生素耐药性的进化压力。

    Antibiotic resistance profile for Neisseria gonorrhoeae and use of same in diagnosis and treatment of gonorrhea
    3.
    发明申请
    Antibiotic resistance profile for Neisseria gonorrhoeae and use of same in diagnosis and treatment of gonorrhea 有权
    淋病奈瑟氏球菌的抗生素耐药性及其在淋病诊断和治疗中的应用

    公开(公告)号：US20120071336A1

    公开(公告)日：2012-03-22

    申请号：US13134252

    申请日：2011-06-02

    申请人： Sergey Balashov ,  Eli Mordechai ,  Martin E. Adelson ,  Scott E. Gygax

    发明人： Sergey Balashov ,  Eli Mordechai ,  Martin E. Adelson ,  Scott E. Gygax

    IPC分类号： C40B30/04 ,  C12Q1/68

    CPC分类号： C12Q1/689 ,  C12Q2600/16

    摘要： The present invention relates to an antibiotic resistance profile for Neisseria gonorrhoeae by assessing the presence of mutations (e.g., SNP) in antibiotic resistant genes that confer bacterial resistance against antibiotics such as penicillin, tetracycline, fluoroquinolones, cephalosporin, macrolides and spectinomycin. There is provided a method and a kit for generating an antibiotic resistance profile for Neisseria gonorrhoeae by utilizing a multiplex PCR to amplify segments of antibiotic-resistant genes, allele-specific primer extension to detect gene mutation, and detection of such gene mutations with gel electrophoresis, capillary electrophoresis, or DNA microarray. The present method provides useful information to physicians relating the antibiotic susceptibility of Neisseria gonorrhoeae against different classes of antibiotics. Relying on this personalized diagnostic tool, physicians can better inform about antibiotic susceptibility and thereby open up medical intervention avenues for treating Neisseria gonorrhoeae with antibiotics. There is provided a therapeutic application of the antibiotic resistance profile that has advantages of: (i) providing a more effective regime for gonorrhea treatment; and (ii) halting the evolutionary pressures towards antibiotic resistance in the Neisseria gonorrhoeae therapy.

    摘要翻译： 本发明涉及通过评估抗生素抗性基因中的突变（例如SNP）的存在，其对抗生素如青霉素，四环素，氟喹诺酮，头孢菌素，大环内酯和壮观霉素提供细菌耐药性，涉及淋病奈瑟氏球菌抗生素抗性谱。 提供了一种通过利用多重PCR扩增抗生素抗性基因片段，等位基因特异性引物延伸以检测基因突变以及用凝胶电泳检测这种基因突变来产生淋病奈瑟氏球菌抗生素抗性谱的方法和试剂盒 ，毛细管电泳或DNA微阵列。 本方法为医生提供了与淋病奈瑟氏球菌对不同类型抗生素的抗生素敏感性有关的有用信息。 依靠这种个性化诊断工具，医生可以更好地了解抗生素敏感性，从而开辟用抗生素治疗淋病奈瑟氏球菌的医疗干预途径。 提供了抗生素抗性谱的治疗应用，其优点在于：（i）为淋病治疗提供更有效的方案; 和（ii）阻止淋病奈瑟氏球菌治疗中抗生素耐药性的进化压力。

    Novel single nucleotide polymorphisms and community-associated methicillin-resistant staphylococcus aureus
    8.
    发明申请
    Novel single nucleotide polymorphisms and community-associated methicillin-resistant staphylococcus aureus 有权
    新型单核苷酸多态性和社区相关耐甲氧西林金黄色葡萄球菌

    公开(公告)号：US20120009572A1

    公开(公告)日：2012-01-12

    申请号：US13068331

    申请日：2011-05-09

    申请人： Scott E. Gygax ,  Sean Chadwick ,  Aditya Prasad ,  Martin E. Adelson ,  Eli Mordechai

    发明人： Scott E. Gygax ,  Sean Chadwick ,  Aditya Prasad ,  Martin E. Adelson ,  Eli Mordechai

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/689 ,  C12Q2600/156

    摘要： The present invention is based on the discovery of novel polymorphisms (SNPs) in the penicillin binding protein (pbp3) gene in Staphylococcus aureus. The presence of G88A and/or G2047A SNPs provides an accurate, reliable biomarker for the presence of Methicillin Resistant Staphylococcus aureus (MRSA), specifically the community-associated MRSA (CA-MRSA). The present invention provides reagents used for detecting the SNPs as well as methods of identifying and using these variants to screen subjects for presence of CA-MRSA. The methods involve isolating a biological sample from a mammal (preferably a human) and testing for the presence of a SNP in the pbp3 gene which is associated with CA-MRSA.

    摘要翻译： 本发明基于在金黄色葡萄球菌中青霉素结合蛋白（pbp3）基因中新型多态性（SNP）的发现。 G88A和/或G2047A SNP的存在为耐甲氧西林金黄色葡萄球菌（MRSA），特别是社区相关MRSA（CA-MRSA）的存在提供了准确，可靠的生物标志物。 本发明提供用于检测SNP的试剂以及鉴定和使用这些变体筛选受试者存在CA-MRSA的方法。 所述方法包括从哺乳动物（优选人）分离生物样品并测试与CA-MRSA相关的pbp3基因中SNP的存在。

    Method of determining types I, II, III, IV or V or methicillin-resistant Staphylococcus aureus (MRSA) in a biological sample
    10.
    发明授权
    Method of determining types I, II, III, IV or V or methicillin-resistant Staphylococcus aureus (MRSA) in a biological sample 有权
    在生物样品中测定I，II，III，IV或V型或耐甲氧西林金黄色葡萄球菌（MRSA）的方法

    公开(公告)号：US08715936B2

    公开(公告)日：2014-05-06

    申请号：US12930663

    申请日：2011-01-13

    申请人： Scott E. Gygax ,  Christina Lim Overmyer ,  Lisa A. DeSalvia ,  Martin E. Adelson ,  Eli Mordechai

    发明人： Scott E. Gygax ,  Christina Lim Overmyer ,  Lisa A. DeSalvia ,  Martin E. Adelson ,  Eli Mordechai

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/04

    CPC分类号： C12Q1/689 ,  A61K31/5377 ,  A61K38/12 ,  A61K38/14 ,  C12Q1/6844 ,  C12Q2600/16

    摘要： Disclosed are diagnostic methods for determining a subtype of methicillin-resistant Staphylococcus aureus (MRSA) in a biological sample of a mammal. Methods include providing a biological sample of the mammal, performing a PCR analysis of the biological sample, and analyzing the PCR amplicons with respect to their sizes so as to determine for type I, type II, type III, type IV or type V MRSA that may be present in the biological sample. Further example embodiments include using at least one mecA primer pair and/or using at least one Staphylococcus aureus nuc primer pair in the PCR analysis. Further disclosed are methods for screening populations for MRSA, and methods of treating a mammal testing positive for Type IV MRSA. Also disclosed are kits for determining a MRSA subtype in a mammal and isolated primers that may be used in the present methods and kits.

    摘要翻译： 公开了用于确定哺乳动物生物样品中耐甲氧西林金黄色葡萄球菌（MRSA）亚型的诊断方法。 方法包括提供哺乳动物的生物样品，对生物样品进行PCR分析，并根据其大小对PCR扩增子进行分析，以确定I型，II型，III型，IV型或V型MRSA， 可能存在于生物样品中。 另外的实施例包括在PCR分析中使用至少一个mecA引物对和/或使用至少一种金黄色葡萄球菌引物对。 进一步披露的是用于筛选MRSA群体的方法以及治疗IV型MRSA阳性的哺乳动物的方法。 还公开了用于测定哺乳动物中MRSA亚型的试剂盒和可用于本发明方法和试剂盒的分离的引物。