发明授权
- 专利标题: Methods and means for obtaining modified phenotypes
- 专利标题(中): 获得修饰表型的方法和手段
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申请号: US13092645申请日: 2011-04-22
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公开(公告)号: US09399777B2公开(公告)日: 2016-07-26
- 发明人: Peter Michael Waterhouse , Ming-Bo Wang
- 申请人: Peter Michael Waterhouse , Ming-Bo Wang
- 申请人地址: AU Campbell
- 专利权人: Commonwealth Scientific & Industrial Research Organisation
- 当前专利权人: Commonwealth Scientific & Industrial Research Organisation
- 当前专利权人地址: AU Campbell
- 代理机构: Buchanan Ingersoll & Rooney PC
- 主分类号: C12N15/113
- IPC分类号: C12N15/113 ; C12N15/82
摘要:
Methods and means are provided for reducing the phenotypic expression of a nucleic acid of interest in eukaryotic cells, particularly in plant cells, by providing aberrant, preferably unpolyadenylated, target-specific RNA to the nucleus of the host cell. Preferably, the unpolyadenylated target-specific RNA is provided by transcription of a chimeric gene comprising a promoter, a DNA region encoding the target-specific RNA, a self-splicing ribozyme and a DNA region involved in 3′ end formation and polyadenylation.
公开/授权文献
- US20120210469A1 METHODS AND MEANS FOR OBTAINING MODIFIED PHENOTYPES 公开/授权日:2012-08-16