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公开(公告)号：US11560571B2
公开(公告)日：2023-01-24
申请号：US17064672
申请日：2020-10-07
IPC分类号： C12N15/82 , C12N15/11 , C12N15/113
摘要： The present invention relates to genetically modified cells that are capable of optimal transgene expression by co-expressing a silencing suppressor whilst at the same time are also capable of silencing a gene, such as a naturally occurring gene of the cell. The present invention also relates to methods of producing the modified cells, as well as relates to processes for obtaining a genetically modified cell with a desired property.
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公开(公告)号：US06492160B1
公开(公告)日：2002-12-10
申请号：US09104337
申请日：1998-06-25
申请人： Andrew David Griffiths , Samuel Cameron Williams , Peter Michael Waterhouse , Ahuva Nissim , Gregory Paul Winter , Kevin Stuart Johnson , Andrew John Hammond Smith
发明人： Andrew David Griffiths , Samuel Cameron Williams , Peter Michael Waterhouse , Ahuva Nissim , Gregory Paul Winter , Kevin Stuart Johnson , Andrew John Hammond Smith
IPC分类号： C12N701
CPC分类号： C40B40/02 , B01J2219/00718 , C07K16/00 , C07K16/18 , C07K16/22 , C07K16/241 , C07K16/28 , C07K16/2812 , C07K16/2863 , C07K16/2866 , C07K16/3007 , C07K16/34 , C07K16/40 , C07K16/4208 , C07K16/44 , C07K16/462 , C07K16/468 , C07K19/00 , C07K2317/21 , C07K2317/24 , C07K2317/55 , C07K2317/622 , C07K2319/00 , C07K2319/02 , C07K2319/41 , C07K2319/735 , C12N15/1037 , C12N15/62 , C12N15/73 , G01N33/53 , G01N33/6857
摘要： Methods, recombinant host cells and kits are disclosed-for the production of members of specific binding pairs (sbp), e.g. antibodies, using display on the surface of secreted replicable genetic display packages (rgdps), e.g. filamentous phage. To produce a library of great diversity recombination occurs between first and second vectors comprising nucleic acid encoding first and second polypeptide chains of sbp members respectively, thereby producing recombinant vectors each encoding both a first and a second polypeptide chain component of a sbp member. The recombination may take place in vitro or intracellularly and may be site-specific, e.g. involving use of the loxP sequence and mutants thereof. Recombination may take place after prior screening or selecting for rgdps displaying sbp members which bind complementary sbp member of interest.
摘要翻译： 公开了方法，重组宿主细胞和试剂盒，用于生产特异性结合对（sbp）的成员，例如。 抗体，使用分泌的可复制遗传显示包（rgdps）的表面上的显示。 丝状噬菌体 为了产生大量多样性的文库，在包含分别编码sbp成员的第一和第二多肽链的核酸的第一和第二载体之间发生重组，从而产生每个编码sbp成员的第一和第二多肽链组分的重组载体。 重组可以在体外或细胞内发生，并且可以是位点特异性的，例如， 涉及使用loxP序列及其突变体。 重组可能在先前筛选或选择显示sbp成员的rgdps之后进行，这些成员绑定了感兴趣的补充sbp成员。
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公开(公告)号：US5733743A
公开(公告)日：1998-03-31
申请号：US307619
申请日：1994-11-18
申请人： Kevin Stuart Johnson , Gregory Paul Winter , Andrew David Griffiths , Andrew John Hammond Smith , Peter Michael Waterhouse
发明人： Kevin Stuart Johnson , Gregory Paul Winter , Andrew David Griffiths , Andrew John Hammond Smith , Peter Michael Waterhouse
IPC分类号： C07K16/18 , C07K16/22 , C07K16/24 , C07K16/28 , C07K16/30 , C07K16/40 , C07K16/42 , C07K16/46 , C12N15/62 , C12P21/06 , C12N1/20 , C12N15/00 , C12N15/63
CPC分类号： C07K16/462 , C07K16/18 , C07K16/22 , C07K16/241 , C07K16/28 , C07K16/2812 , C07K16/2863 , C07K16/2866 , C07K16/3007 , C07K16/40 , C07K16/4208 , C07K16/468 , C12N15/62 , B01J2219/00718 , C07K2317/24 , C07K2317/55 , C07K2317/622 , C07K2319/00 , C07K2319/02 , C07K2319/41 , C07K2319/735
摘要： Methods, recombinant host cells and kits are disclosed for the production of members of specific binding pairs (sbp), e.g. antibodies, using display on the surface of secreted replicable genetic display packages (rgdps), e.g. filamentous phage. To produce a library of great diversity, recombination occurs between first and second vectors comprising nucleic acid encoding first and second polypeptide chains of sbp members respectively, thereby producing recombinant vectors each encoding both a first and a second polypeptide chain component of an sbp member. The recombination may take place in vitro or intracellularly and may be site-specific, e.g. involving use of the loxP sequence and mutants thereof. Recombination may take place after prior screening or selecting for rgdps displaying sbp members which bind complementary sbp member of interest.
摘要翻译： PCT No.PCT / GB93 / 00605 Sec。 371日期：1994年11月18日 102（e）1994年11月18日日期PCT 1993年3月24日PCT公布。 WO93 / 19172 PCT出版物 1993年9月30日的日期公开了用于产生特异性结合对（sbp）成员的重组宿主细胞和试剂盒，例如， 抗体，使用分泌的可复制遗传显示包（rgdps）的表面上的显示。 丝状噬菌体 为了产生大量多样性的文库，在包含分别编码sbp成员的第一和第二多肽链的核酸的第一和第二载体之间进行重组，由此产生每个编码sbp成员的第一和第二多肽链组分的重组载体。 重组可以在体外或细胞内发生，并且可以是位点特异性的，例如， 涉及使用loxP序列及其突变体。 重组可能在先前筛选或选择显示sbp成员的rgdps之后进行，这些成员绑定了感兴趣的补充sbp成员。
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公开(公告)号：US09441239B2
公开(公告)日：2016-09-13
申请号：US11364183
申请日：2006-03-01
CPC分类号： C12N15/8218 , A01K2217/05 , A61K48/00 , C12N15/8203 , C12N15/8247 , C12N15/8282 , C12N15/8283
摘要： Methods and means are provided for reducing the phenotypic expression of a nucleic acid of interest in eucaryotic cells, particularly in plant cells, by introducing chimeric genes encoding sense and antisense RNA molecules directed towards the target nucleic acid, which are capable of forming a double stranded RNA region by base-pairing between the regions with sense and antisense nucleotide sequence or by introducing the RNA molecules themselves. Preferably, the RNA molecules comprises simultaneously both sense and antisense nucleotide sequence.
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公开(公告)号：US09399777B2
公开(公告)日：2016-07-26
申请号：US13092645
申请日：2011-04-22
IPC分类号： C12N15/113 , C12N15/82
CPC分类号： C12N15/8218 , C12N9/2405 , C12N15/8216 , C12N15/8247 , C12N15/8283 , C12Y302/01031
摘要： Methods and means are provided for reducing the phenotypic expression of a nucleic acid of interest in eukaryotic cells, particularly in plant cells, by providing aberrant, preferably unpolyadenylated, target-specific RNA to the nucleus of the host cell. Preferably, the unpolyadenylated target-specific RNA is provided by transcription of a chimeric gene comprising a promoter, a DNA region encoding the target-specific RNA, a self-splicing ribozyme and a DNA region involved in 3′ end formation and polyadenylation.
摘要翻译： 提供了通过向宿主细胞核提供异常的，优选未多聚腺苷酸化的靶特异性RNA来减少真核细胞，特别是植物细胞中目的核酸的表型表达的方法和手段。 优选地，未多聚腺苷酸化的靶特异性RNA通过包含启动子，编码靶特异性RNA的DNA区，自剪接核酶和参与3'端形成和多聚腺苷酸化的DNA区的嵌合基因的转录来提供。
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公开(公告)号：US20140193856A1
公开(公告)日：2014-07-10
申请号：US14137737
申请日：2013-12-20
IPC分类号： C12N15/113
CPC分类号： C12N15/113 , A01K2217/05 , A61K48/00 , C12N9/1051 , C12N9/127 , C12N9/503 , C12N15/111 , C12N15/1131 , C12N15/63 , C12N15/69 , C12N15/8216 , C12N15/8218 , C12N15/8283 , C12N15/85 , C12N2310/111 , C12N2310/14 , C12N2310/531 , C12N2320/10 , C12N2320/30 , C12N2330/30 , C12N2330/50 , C12N2330/51 , C12N2800/108 , C12N2830/00 , C12N2830/002 , C12N2830/15 , C12N2830/38 , C12N2830/42 , C12N2830/55 , C12N2830/60 , C12N2840/20
摘要： The present invention relates generally to a method of modifying gene expression and to synthetic genes for modifying endogenous gene expression in a cell, tissue or organ of a transgenic organism, in particular a transgenic animal or plant. More particularly, the present invention utilizes recombinant DNA technology to post-transcriptionally modify or modulate the expression of a target gene in a cell, tissue organ or whole organism, thereby producing novel phenotypes. Novel synthetic genes and genetic constructs which are capable of repressing delaying or otherwise reducing the expression of an endogenous gene or target gene in an organism when introduced thereto are also provided.
摘要翻译： 本发明一般涉及修饰基因表达的方法和用于修饰转基因生物体，特别是转基因动物或植物的细胞，组织或器官中的内源基因表达的合成基因。 更具体地，本发明利用重组DNA技术转录后修饰或调节细胞，组织器官或整个生物体中靶基因的表达，从而产生新的表型。 还提供了新的合成基因和遗传构建体，其能够抑制在引入生物体时延迟或以其它方式减少生物体中的内源基因或靶基因的表达。
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公开(公告)号：US08598332B1
公开(公告)日：2013-12-03
申请号：US09287632
申请日：1999-04-07
CPC分类号： C12N15/8218 , C12N15/8247
摘要： Methods and means are provided for reducing the phenotypic expression of a nucleic acid of interest in eucaryotic cells, particularly in plant cells, by introducing chimeric genes encoding sense and antisense RNA molecules directed towards the target nucleic acid, which are capable of forming a double stranded RNA region by base-pairing between the regions with sense and antisense nucleotide sequence or by introducing the RNA molecules themselves. Preferably, the RNA molecules comprises simultaneously both sense and antisense nucleotide sequence.
摘要翻译： 提供了通过引入编码针对靶核酸的正义和反义RNA分子的嵌合基因来降低真核细胞，特别是植物细胞中感兴趣的核酸的表型表达的方法和手段，其能够形成双链 RNA区域通过有义和反义核苷酸序列的区域之间的碱基配对或通过引入RNA分子本身。 优选地，RNA分子同时包含有义和反义核苷酸序列。
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公开(公告)号：US20120270315A1
公开(公告)日：2012-10-25
申请号：US13474539
申请日：2012-05-17
IPC分类号： C12N15/113 , C12N5/10
CPC分类号： C12N15/8218 , C12N9/2405 , C12N15/8216 , C12N15/8247 , C12N15/8283 , C12Y302/01031
摘要： Methods and means are provided for reducing the phenotypic expression of a nucleic acid of interest in eukaryotic cells, particularly in plant cells, by providing aberrant, preferably unpolyadenylated, target-specific RNA to the nucleus of the host cell. Preferably, the unpolyadenylated target-specifc RNA is provided by transcription of a chimeric gene comprising a promoter, a DNA region encoding the target-specific RNA, a self-splicing ribozyme and a DNA region involved in 3′ end formation and polyadenylation.
摘要翻译： 提供了通过向宿主细胞核提供异常的，优选未多聚腺苷酸化的靶特异性RNA来减少真核细胞，特别是植物细胞中目的核酸的表型表达的方法和手段。 优选地，通过转录包含启动子，编码靶特异性RNA的DNA区，自剪接核酶和参与3'末端形成和多聚腺苷酸化的DNA区的嵌合基因来提供未多聚腺苷酸化的靶特异性RNA。
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公开(公告)号：US20080044906A1
公开(公告)日：2008-02-21
申请号：US10571384
申请日：2004-09-10
申请人： Peter Michael Waterhouse , Linda Jane Lockett , Ming-Bo Wang , Timothy James Doran , Robert John Moore , Gerald Wayne Both
发明人： Peter Michael Waterhouse , Linda Jane Lockett , Ming-Bo Wang , Timothy James Doran , Robert John Moore , Gerald Wayne Both
CPC分类号： C12N15/113 , A01K2217/05 , A01K2217/058 , C12N15/11 , C12N15/1131 , C12N2310/11 , C12N2310/111 , C12N2310/3519 , C12N2310/53 , C12N2799/027
摘要： Methods and means for efficiently downregulating the expression of a target gene of interest in cell from an organism that is an animal, fungus and protist. The invention provides chimeric nucleic acid molecules for downregulating target genes. The invention also provides modified cells and organisms comprising the chimeric nucleic acid molecules and compositions comprising the chimeric molecules.
摘要翻译： 用于从动物，真菌和原生生物的生物体中有效地下调目标基因在细胞中的表达的方法和手段。 本发明提供用于下调靶基因的嵌合核酸分子。 本发明还提供了修饰的细胞和生物体，其包含嵌合核酸分子和包含嵌合分子的组合物。
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公开(公告)号：US6140471A
公开(公告)日：2000-10-31
申请号：US50783
申请日：1998-03-30
申请人： Kevin Stuart Johnson , Gregory Paul Winter , Andrew David Griffiths , Andrew John Hammond Smith , Peter Michael Waterhouse
发明人： Kevin Stuart Johnson , Gregory Paul Winter , Andrew David Griffiths , Andrew John Hammond Smith , Peter Michael Waterhouse
IPC分类号： C07K16/18 , C07K16/22 , C07K16/24 , C07K16/28 , C07K16/30 , C07K16/40 , C07K16/42 , C07K16/46 , C12N15/62 , C12P21/08
CPC分类号： C12N15/62 , C07K16/18 , C07K16/22 , C07K16/241 , C07K16/28 , C07K16/2812 , C07K16/2863 , C07K16/2866 , C07K16/3007 , C07K16/40 , C07K16/4208 , C07K16/462 , C07K16/468 , B01J2219/00718 , C07K2317/24 , C07K2317/55 , C07K2317/622 , C07K2319/00 , C07K2319/02 , C07K2319/41 , C07K2319/735
摘要： Methods, recombinant host cells and kits are disclosed for the production of members of specific binding pairs (sbp), e.g. antibodies, using display on the surface of secreted replicable genetic display packages (rgdps), e.g. filamentous phage. To produce a library of great diversity, recombination occurs between first and second vectors comprising nucleic acid encoding first and second polypeptide chains of sbp members respectively, thereby producing recombinant vectors each encoding both a first and a second polypeptide chain component of an sbp member. The recombination may take place in vitro or intracellularly and may be site-specific, e.g. involving use of the loxP sequence and mutants thereof. Recombination may take place after prior screening or selecting for rgdps displaying sbp members which bind complementary sbp member of interest.
摘要翻译： 公开了用于产生特异性结合对（sbp）成员的重组宿主细胞和试剂盒，例如， 抗体，使用分泌的可复制遗传显示包（rgdps）的表面上的显示。 丝状噬菌体 为了产生大量多样性的文库，在包含分别编码sbp成员的第一和第二多肽链的核酸的第一和第二载体之间进行重组，由此产生每个编码sbp成员的第一和第二多肽链组分的重组载体。 重组可以在体外或细胞内发生，并且可以是位点特异性的，例如， 涉及使用loxP序列及其突变体。 重组可能在先前的筛选或选择rgdps显示sbp成员之后发生，该成员绑定了感兴趣的补充sbp成员。
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