A positioning method and a positioning system based on light intensity are provided. The positioning system comprises a lighting system, a sense feedback device and a positioning module. The lighting system comprises at least three point light sources and sequentially adjusts luminance of these point light sources to light up a target. The sense feedback device is disposed on the target and used to collect light intensity information of the light projected on the target by the lighting system. The positioning module calculates a distance between the target and each of the point light sources based on the light intensity information and calculates a positioning location of the target based on the locations of the point light sources and the distances between the target and the point light sources.