A micro fluidic apparatus includes a sample chamber that divides a sample into a first fluid layer and a second fluid layer due to a centrifugal force; a first enriching unit a that receives the first fluid layer from the sample chamber, forms a first complex of a first fine particle and the first target material, and separates the first complex from the first fluid layer using a density difference; and a second enriching unit that receives the second fluid layer from the sample chamber, forms a second complex of a second fine particle and the second target material, and separates the second complex from the second fluid layer using a density difference.