发明授权
US09448237B2 Modified tyrosine amino acids, peptides containing them, and their use for detecting hydrolase enzyme activity
有权
修饰的酪氨酸氨基酸，含有它们的肽，以及它们用于检测水解酶活性的用途
- 专利标题： Modified tyrosine amino acids, peptides containing them, and their use for detecting hydrolase enzyme activity
- 专利标题（中）： 修饰的酪氨酸氨基酸，含有它们的肽，以及它们用于检测水解酶活性的用途
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申请号： US14118920申请日： 2012-05-18
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公开(公告)号： US09448237B2公开(公告)日： 2016-09-20
- 发明人: Robby Ruijtenbeek , Jeroen van Ameijde , Robertus Matthias Joseph Liskamp
- 申请人： Robby Ruijtenbeek , Jeroen van Ameijde , Robertus Matthias Joseph Liskamp
- 申请人地址： NL 'S-Hertogenbosch NL Utrecht
- 专利权人： PAMGENE B.V.,UNIVERSITEIT UTRECHT HOLDING B.V.
- 当前专利权人： PAMGENE B.V.,UNIVERSITEIT UTRECHT HOLDING B.V.
- 当前专利权人地址： NL 'S-Hertogenbosch NL Utrecht
- 代理机构： Amster, Rothstein & Ebenstein LLP
- 优先权： EP11166908 20110520
- 国际申请： PCT/EP2012/059293 WO 20120518
- 国际公布： WO2012/159997 WO 20121129
- 主分类号： C07K2/00
- IPC分类号： C07K2/00 ; C07K14/00 ; G01N33/573 ; C07C305/24 ; C07F9/12 ; C12Q1/42 ; C07F9/08
摘要：
The present invention relates to a method and tools which allow the kinetic monitoring of hydrolase enzyme activity. The present invention also aims to provide synthetic peptides and peptide residues modified such that they can be used in assays for monitoring hydrolase enzyme activity, and preferably phosphatase activity. Also claimed are modified tyrosine amino acid derivatives of formula (IV), wherein Y is chosen from —POy2− with y equal to 2 or 3 and esters thereof, —SOZ− with z equal to 2 or 3 and esters thereof, a carbohydrate or carbohydrate derivatives; and; wherein X1 and/or X2 is selected from —N3, —NO2, —CI, or —CN, —SCN, a C1 to C8 alkyl, or H, wherein either X1 or X2 is not H.
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