专利检索 ap:"Jeroen van Ameijde" 第 1 页

1.

发明授权
Modified tyrosine amino acids, peptides containing them, and their use for detecting hydrolase enzyme activity 有权
标题翻译：修饰的酪氨酸氨基酸，含有它们的肽，以及它们用于检测水解酶活性的用途

公开(公告)号：US09448237B2

公开(公告)日：2016-09-20

申请号：US14118920

申请日：2012-05-18

申请人： Robby Ruijtenbeek , Jeroen van Ameijde , Robertus Matthias Joseph Liskamp

发明人： Robby Ruijtenbeek , Jeroen van Ameijde , Robertus Matthias Joseph Liskamp

IPC分类号： C07K2/00 , C07K14/00 , G01N33/573 , C07C305/24 , C07F9/12 , C12Q1/42 , C07F9/08

CPC分类号： G01N33/573 , C07C305/24 , C07F9/08 , C07F9/12 , C12Q1/42

摘要： The present invention relates to a method and tools which allow the kinetic monitoring of hydrolase enzyme activity. The present invention also aims to provide synthetic peptides and peptide residues modified such that they can be used in assays for monitoring hydrolase enzyme activity, and preferably phosphatase activity. Also claimed are modified tyrosine amino acid derivatives of formula (IV), wherein Y is chosen from —POy2− with y equal to 2 or 3 and esters thereof, —SOZ− with z equal to 2 or 3 and esters thereof, a carbohydrate or carbohydrate derivatives; and; wherein X1 and/or X2 is selected from —N3, —NO2, —CI, or —CN, —SCN, a C1 to C8 alkyl, or H, wherein either X1 or X2 is not H.