Invention Grant
- Patent Title: Methods and compositions for labeling nucleic acids
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Application No.: US13972365Application Date: 2013-08-21
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Publication No.: US09512465B2Publication Date: 2016-12-06
- Inventor: Kyle R. Gee , Brian Agnew , Adrian Salic , Timothy J. Mitchison
- Applicant: President and Fellows of Harvard College , Life Technologies Corporation
- Applicant Address: US CA Carlsbad US MA Cambridge
- Assignee: Life Technologies Corporation,President and Fellows of Harvard College
- Current Assignee: Life Technologies Corporation,President and Fellows of Harvard College
- Current Assignee Address: US CA Carlsbad US MA Cambridge
- Agency: Choate, Hall & Stewart, LLP
- Agent Charles E. Lyon; Xiaodong Li
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; G01N33/50 ; G01N33/58

Abstract:
The present invention relates to methods for the labeling of nucleic acid polymers in vitro and in vivo. Certain methods are provided that include a [3+2] cycloaddition between a nucleotide analogue incorporated into a nucleic acid polymer and a reagent attached to a label. Other methods are provided that include a Staudinger ligation between a nucleotide analogue incorporated into a nucleic acid polymer and a reagent comprising a substituted triarylphosphine attached to a label. Such methods do not require fixation and denaturation and therefore can be applied to the labeling of nucleic acid polymers in living cells and in organisms. Also provided are methods for measuring cellular proliferation. In these methods, the amount of label incorporated into the DNA is measured as an indication of cellular proliferation. The methods of the invention can be used in a wide variety of applications including clinical diagnosis of diseases and disorders in which cellular proliferation is involved, toxicity assays, and as a tool for the study of chromosomes' ultrastructures.
Public/Granted literature
- US20140065605A1 METHODS AND COMPOSITIONS FOR LABELING NUCLEIC ACIDS Public/Granted day:2014-03-06
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