- 专利标题: Protein and nucleic acid delivery vehicles, components and mechanisms thereof
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申请号: US14322097申请日: 2014-07-02
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公开(公告)号: US09523101B2公开(公告)日: 2016-12-20
- 发明人: Venigalla B. Rao
- 申请人: The Catholic University of America
- 申请人地址: US DC Washington
- 专利权人: The Catholic University of America
- 当前专利权人: The Catholic University of America
- 当前专利权人地址: US DC Washington
- 代理机构: Miles & Stockbridge PC
- 代理商 Ajay A. Jagtiani
- 主分类号: C12N15/86
- IPC分类号: C12N15/86 ; C12N15/87 ; C07K14/005 ; C12N7/00 ; C12N15/88 ; A61K47/48 ; A61K31/7088 ; A61K31/711 ; A61K48/00
摘要:
Complex viruses are assembled from simple protein subunits by sequential and irreversible assembly. During genome packaging in bacteriophages, a powerful molecular motor assembles at the special portal vertex of an empty prohead to initiate packaging. An aspect of the invention relates to the phage T4 packaging machine being highly promiscuous, translocating DNA into finished phage heads as well as into proheads. Single motors can force exogenous DNA into phage heads at the same rate as into proheads and phage heads undergo repeated initiations, packaging multiple DNA molecules into the same head. This shows that the phage DNA packaging machine has unusual conformational plasticity, powering DNA into an apparently passive capsid receptacle, including the highly stable virus shell, until it is full. These features allow for the design of a novel class of nanocapsid delivery vehicles.