Systems and techniques are described for a path maximum transmission unit (MTU) discovery method that allows the sender of IP packets to discover the MTU of packets that it is sending over a conduit to a given destination. The MTU is the largest packet that can be sent through the network along a path without requiring fragmentation. The path MTU discovery method actively probes each sending path of each conduit with fragmentation enabled to determine a current MTU and accordingly increase or decrease the conduit MTU. The path MTU discovery process is resilient to errors and supports retransmission if packets are lost in the discovery process. The path MTU discovery process is dynamically adjusted at a periodic rate to adjust to varying network conditions.