Invention Grant
- Patent Title: Method for constructing functional nucleic acid molecule, and nucleic acid combination to be used in said method
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Application No.: US14381681Application Date: 2013-03-01
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Publication No.: US09598713B2Publication Date: 2017-03-21
- Inventor: Hiroshi Abe , Yoshihiro Ito , Hideto Maruyama
- Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY
- Applicant Address: JP Saitama
- Assignee: Japanese Science and Technology Agency
- Current Assignee: Japanese Science and Technology Agency
- Current Assignee Address: JP Saitama
- Agency: Amster, Rothstein & Ebenstein LLP
- Priority: JP2012-047367 20120302
- International Application: PCT/JP2013/055732 WO 20130301
- International Announcement: WO2013/129663 WO 20130906
- Main IPC: C12P19/34
- IPC: C12P19/34 ; C12N15/10 ; C12N15/113 ; C12N15/11
Abstract:
A method for constructing a functional nucleic acid molecule comprising 1 or 2 nucleic acid strands, wherein 2 or more fragments having at corresponding ends a functional group pair that can mutually couple through a chemical reaction are introduced into a cell, and a functional nucleic acid molecule comprising 1 or 2 nucleic acid strands is formed by ligating mutually the fragments through a reaction between the functional groups in the cell.
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