公开(公告)号：US20150118713A1

公开(公告)日：2015-04-30

申请号：US14381681

申请日：2013-03-01

Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY

Inventor： Hiroshi Abe ,  Yoshihiro Ito ,  Hideto Maruyama

IPC: C12P19/34

CPC classification number: C12P19/34 ,  C12N15/1068 ,  C12N15/111 ,  C12N15/113 ,  C12N2310/14 ,  C12N2310/315 ,  C12N2330/30

Abstract: A method for constructing a functional nucleic acid molecule comprising 1 or 2 nucleic acid strands, wherein 2 or more fragments having at corresponding ends a functional group pair that can mutually couple through a chemical reaction are introduced into a cell, and a functional nucleic acid molecule comprising 1 or 2 nucleic acid strands is formed by ligating mutually the fragments through a reaction between the functional groups in the cell.

Abstract translation: 构建包含1或2个核酸链的功能性核酸分子的方法，其中在相应末端具有可通过化学反应相互耦合的官能团对的2个或更多个片段被引入细胞中，并且功能性核酸分子 通过在细胞中的官能团之间的反应相互连接片段而形成包含1或2个核酸链。

公开(公告)号：US09598713B2

公开(公告)日：2017-03-21

申请号：US14381681

申请日：2013-03-01

Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY

Inventor： Hiroshi Abe ,  Yoshihiro Ito ,  Hideto Maruyama

IPC: C12P19/34 ,  C12N15/10 ,  C12N15/113 ,  C12N15/11

CPC classification number: C12P19/34 ,  C12N15/1068 ,  C12N15/111 ,  C12N15/113 ,  C12N2310/14 ,  C12N2310/315 ,  C12N2330/30

Abstract: A method for constructing a functional nucleic acid molecule comprising 1 or 2 nucleic acid strands, wherein 2 or more fragments having at corresponding ends a functional group pair that can mutually couple through a chemical reaction are introduced into a cell, and a functional nucleic acid molecule comprising 1 or 2 nucleic acid strands is formed by ligating mutually the fragments through a reaction between the functional groups in the cell.

公开(公告)号：US10570385B2

公开(公告)日：2020-02-25

申请号：US15505656

申请日：2015-08-26

Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY

Inventor： Hiroshi Abe ,  Hideto Maruyama

IPC: C12N15/09 ,  C07H21/00 ,  C12P19/34 ,  C12Q1/6806 ,  C12Q1/6811 ,  C07H1/00 ,  C12Q1/68

Abstract: A non-enzymatic method is provided for binding a first nucleic acid chain to a second nucleic acid chain without introducing a sulfur atom into the combined nucleic acid chain, the method comprising reacting a first nucleic acid chain having a phosphorothioate group at the 3′ or 5′ terminus with a second nucleic acid chain having a hydroxyl group or an amino group at the 3′ or 5′ terminus in the presence of an electrophile that has a leaving group and is configured to leave the leaving group and bind to a sulfur atom of the phosphorothioate group of the first nucleic acid chain at the site to which the leaving group had been bound, and remove the sulfur atom from the phosphorothioate group of the first nucleic acid chain and a hydrogen atom from the hydroxyl group or from the amino group of the second nucleic acid chain via a nucleophilic substitution with an oxygen atom of the hydroxyl group or a nitrogen atom of the amino group of the second nucleic acid chain, and thereby form a bond between a phosphorus atom of the phosphate group of the first nucleic acid chain and the oxygen atom or the nitrogen atom of the second nucleic acid chain. Examples of structures produced by the binding method are shown below.