An image sensor including a color filter and a method of manufacturing the image sensor are provided. The image sensor includes a light-sensing layer configured to detect incident light, and convert the incident light to an electrical signal. The image sensor further includes a color filter layer disposed on the light-sensing layer, the color filter layer including color filters, each of the color filters being configured to transmit, among the incident light, light in a wavelength band to the light-sensing layer. The image sensor further includes an isolation layer disposed between the color filters, the isolation layer being configured to optically isolate the color filters from each other. An upper portion of each of the color filters has a cylindrical shape, and a lower portion of each of the color filters has a hemispherical shape.