Invention Grant
- Patent Title: Host cells and method for making acrylate and precursors thereof using an odd-numbered alkane feedstock
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Application No.: US14344899Application Date: 2012-10-17
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Publication No.: US09657317B2Publication Date: 2017-05-23
- Inventor: Joshua A. Silverman , Tom Purcell , Jon Edward Ness , Effendi Leonard
- Applicant: Calysta, Inc.
- Applicant Address: US CA Menlo Park
- Assignee: Calysta, Inc.
- Current Assignee: Calysta, Inc.
- Current Assignee Address: US CA Menlo Park
- Agency: Seed IP Law Group LLP
- International Application: PCT/US2012/060671 WO 20121017
- International Announcement: WO2013/059362 WO 20130425
- Main IPC: C12P7/42
- IPC: C12P7/42 ; C12P19/32 ; C12P7/40 ; C12N15/52

Abstract:
Provided herein is an alkane-metabolizing cell that is unable to convert propionyl-CoA into methylmalonyl-CoA or 2-metylcitrate synthase. Depending on which enzymes are present in the cell, the cell can produce acrylate or a precursor for the same (e.g., propionate, 3-hydroxypropionyl-CoA, 3-hydroxypropionate, acrylyl-CoA) that can be readily converted to acrylate enzymatically (e.g., in the cell) or by chemical treatment. In one embodiment, the cell may contain a cytochrome P450 or alkane oxidase enzyme that allows the production of 3-hydroxypropionyl-CoA, which can be readily converted to 3-hydroxypropionate. In order to make such compounds, the cell may be grown in the presence of an odd-numbered chain alkane (e.g., pentane or heptane), although another odd-numbered chain alkane may be used. In another embodiment, the cell may contain acyl-CoA oxidase, enoyl-CoA hydratase, and hydrolase.
Public/Granted literature
- US20150087036A1 Host Cells and Method for Making Acrylate and Precursors Thereof Using an Odd-Numbered Alkane Feedstock Public/Granted day:2015-03-26
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