A carbon fiber rim comprises a circular rim and a heat conduction layer. The circular rim comprises carbon fiber composites, and has two opposite side surfaces facing two brake pads and two opposite hooks extending outward radially to hold tires. The heat conduction layer is arranged on the side surface of the circular rim for forming a braking region facing the brake pad. The heat conduction layer comprises a porous or mesh heat conduction material. A bicycle including the aforementioned carbon fiber rim and the manufacturing method of the carbon fiber rim are also disclosed here. The carbon fiber rim can prevent distortion due to frictional heat when braking.