A display panel and a display device applying the same are provided. The display panel includes a first substrate, a second substrate, and a display medium disposed between the first substrate and the second substrate. The first substrate includes a first conductive layer having a first line width and a second conductive layer having a second line width smaller than the first line width. A first spacing is defined by a first sidewall of the second conductive layer and a second sidewall, located on the same side as the first sidewall, of the first conductive layer. A second spacing is defined by a third sidewall of the second conductive layer opposite to the first sidewall and a fourth sidewall, located on the same side as the third sidewall, of the first conductive layer. The first spacing is larger than the second spacing.