A color separation device changes a light path according to the wavelengths of incident light and an image sensor has improved light utilization efficiency by using the color separation device. The color separation device may include a first element having a first refractive index that varies according to wavelengths of light along a first refractive index distribution curve, and a second element having a second refractive index that varies according to wavelengths of light along a second refractive index distribution curve, the second refractive index distribution curve being different from the first refractive index distribution curve. The color separation device may be manufactured by simply joining two elements, namely, the first and second elements, together and thus may be more easily manufactured and perform more effective color separation.