There is provided an anti-reflection film that has an excellent reflection characteristic (low reflectivity) in a wide spectrum and has an excellent reflection hue that is close to neutral, and a method by which such anti-reflection film can be produced with high productivity and at a low cost. An anti-reflection film according to the present invention includes: a substrate; and a medium-refractive index layer, a high-refractive index layer, and a low-refractive index layer in the stated order from a substrate side. A refractive index nS of the substrate, a refractive index nM of the medium-refractive index layer, and a refractive index nH of the high-refractive index layer satisfy the following expression (1): n H - 1 n H + 1 - 1 - 4 ⁢ n M 2 ⁢ n S n M 2 ⁡ ( 1 + n S ) 2 - ( 1 - n M 2 ) ⁢ ( n M 2 - n S 2 ) ≧ 0.02 ( 1 ) where the refractive index nS of the substrate, the refractive index nM of the medium-refractive index layer, and the refractive index nH of the high-refractive index layer have a relationship of nH>nM>nS.