A microlens array substrate includes a substrate having a surface in which a concave portion is provided, a lens layer that is provided so as to fill the concave portion, and an optical path length adjustment layer that is provided so as to cover the lens layer. A flat portion is disposed in a central portion of the concave portion. A refractive index of the lens layer is higher than a refractive index of the substrate, and a refractive index of the optical path length adjustment layer is higher than a refractive index of the substrate and is equal to or lower than a refractive index of the lens layer.