- 专利标题: Method of producing retinal pigment epithelial cell sheet
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申请号: US14001108申请日: 2012-02-24
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公开(公告)号: US09902933B2公开(公告)日: 2018-02-27
- 发明人: Masayo Takahashi , Satoshi Okamoto , Hiroyuki Kamao
- 申请人: Masayo Takahashi , Satoshi Okamoto , Hiroyuki Kamao
- 申请人地址: JP Wako
- 专利权人: RIKEN
- 当前专利权人: RIKEN
- 当前专利权人地址: JP Wako
- 代理机构: Leydig, Voit & Mayer, Ltd.
- 优先权: JP2011-040130 20110225
- 国际申请: PCT/JP2012/054631 WO 20120224
- 国际公布: WO2012/115244 WO 20120830
- 主分类号: C12N5/079
- IPC分类号: C12N5/079 ; G01N33/50 ; A61K35/00
摘要:
The present invention provides a method of producing a cell sheet including the following steps(1) seeding and culturing retinal pigment epithelial cells on a collagen gel to form a cell sheet composed of the retinal pigment epithelial cells, and(2) degrading the collagen gel with collagenase to detach the cell sheet composed of the retinal pigment epithelial cells, and the like.