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    Method of producing human retinal pigment epithelial cells
    3.
    发明授权
    Method of producing human retinal pigment epithelial cells 有权

    公开(公告)号：US10519422B2

    公开(公告)日：2019-12-31

    申请号：US13408642

    申请日：2012-02-29

    申请人： Masayo Takahashi ,  Satoshi Okamoto ,  Noriko Sakai

    发明人： Masayo Takahashi ,  Satoshi Okamoto ,  Noriko Sakai

    IPC分类号： C12N5/079 ,  A61K35/30

    摘要： The invention relates to a method of producing a retinal pigment epithelial cell from a human pluripotent stem cell, and a method of treating or preventing a retinal disease by using the produced cell. The retinal pigment epithelial cell is prepared by (a) inducing differentiation of a human pluripotent stem cell into a pigment cell by adhesion cultivation of a human pluripotent stem cell in a medium containing a Nodal signal inhibitor and a Wnt signal inhibitor in the absence of a feeder cell to give a culture containing the pigment cell, (b) subjecting the obtained culture to further adhesion culture to give a culture containing a pigment cell colony, and (c) isolating the pigment cell from the obtained culture and culturing the cell to give a retinal pigment epithelial cell.

    Method of producing lens cell and lens cell obtained by the method
    4.
    发明申请
    Method of producing lens cell and lens cell obtained by the method 审中-公开
    通过该方法获得的透镜细胞和透镜细胞的制造方法

    公开(公告)号：US20070037282A1

    公开(公告)日：2007-02-15

    申请号：US10551378

    申请日：2004-03-22

    申请人： Masayo Takahashi ,  Yoshiki Sasai ,  Hiroshi Kawasaki ,  Sotaro Ooto

    发明人： Masayo Takahashi ,  Yoshiki Sasai ,  Hiroshi Kawasaki ,  Sotaro Ooto

    IPC分类号： C12N5/08

    CPC分类号： C12N5/0621 ,  C12N2501/115 ,  C12N2506/02

    摘要： A method of producing a lens cell is disclosed. The method includes: an ES cell maintenance step of maintaining an ES cell by using a medium containing a fibroblast growth factor FGF-2 at a concentration of 2 ng/ml to 50 ng/ml; and a differentiation inducing step, carried out after the ES cell maintenance step, of inducing differentiation of the ES cell into a lens cell by implanting and culturing the ES cell on a mouse-skull-cell PA6 at a cell density of 2 colonies/cm2 to 6.5 colonies/cm2. In one embodiment, a washing step, carried out between the ES cell maintenance step and the differentiation inducing step, of washing the maintained ES cell once with an ES differentiation medium is included.

    摘要翻译： 公开了一种制造透镜单元的方法。 该方法包括：通过使用浓度为2ng / ml至50ng / ml的含有成纤维细胞生长因子FGF-2的培养基来维持ES细胞的ES细胞维持步骤; 以及在ES细胞维持步骤之后进行的分化诱导步骤，通过以2个菌落/ cm 2的细胞密度将ES细胞植入和培养到小鼠颅骨细胞PA6上来诱导ES细胞分化成晶状体细胞 2至6.5个菌落/ cm 2。 在一个实施方案中，包括在ES细胞维持步骤和分化诱导步骤之间进行的洗涤步骤，用ES分化培养基洗涤维持的ES细胞一次。

    METHOD FOR PRODUCING CELL SHEET
    5.
    发明申请
    METHOD FOR PRODUCING CELL SHEET 审中-公开
    生产细胞片的方法

    公开(公告)号：US20130108590A1

    公开(公告)日：2013-05-02

    申请号：US13697180

    申请日：2011-05-10

    申请人： Masayo Takahashi ,  Tsutomu Yasukawa

    发明人： Masayo Takahashi ,  Tsutomu Yasukawa

    IPC分类号： C12N5/071

    CPC分类号： C12N5/0602 ,  A61L27/3808 ,  A61L27/3869 ,  A61L2430/16

    摘要： The present invention provides a method for producing a cell sheet, including(1) a step of preparing mammal-derived cells, and (2) a step of seeding the prepared cells on a plane substrate in high density, and culturing the cells, a cell sheet produced by the method, a material for transplantation, Bruch's membrane and the like.

    摘要翻译： 本发明提供一种细胞片的制造方法，其包括（1）制备哺乳动物来源的细胞的步骤，（2）将制备的细胞以高密度接种在平面基质上并培养细胞的步骤 通过该方法生产的细胞片，用于移植的材料，Bruch's膜等。

    Method of isolating adult mammalian CNS-derived progenitor stem cells using density gradient centrifugation
    6.
    发明授权
    Method of isolating adult mammalian CNS-derived progenitor stem cells using density gradient centrifugation 失效
    使用密度梯度离心法分离成年哺乳动物CNS来源的祖细胞干细胞的方法

    公开(公告)号：US06767738B1

    公开(公告)日：2004-07-27

    申请号：US09913192

    申请日：2002-02-12

    申请人： Fred H. Gage ,  Theo Palmer ,  Francis G. Safar ,  Jun Takahashi ,  Masayo Takahashi

    发明人： Fred H. Gage ,  Theo Palmer ,  Francis G. Safar ,  Jun Takahashi ,  Masayo Takahashi

    IPC分类号： C12N502

    CPC分类号： C12N5/0623 ,  A61K35/12 ,  A61K48/00 ,  C12N2501/115 ,  C12N2510/00

    摘要： The present invention is directed to methods of repairing damaged or diseased, specialized or differentiated tissue in mature animals, particularly neuronal tissue such as retinas. In particular, the invention relates to transplantation of adult, hippocampus-derived progenitor cells into a selected neural tissue site of a recipient. These cells can functionally integrate into mature and immature neural tissue. The invention encompasses, in one aspect, repopulating a retina of a dystrophic animal with neurons, by injecting clonally derived, adult central nervous system derived stem cells (ACSC) derived from a healthy donor animal into an eye of the dystrophic recipient. Herein disclosed is the first successful and stable integration of clonally derived ACSC into same-species but different strain recipients (e. g., Fischer rat-derived adult hippocampal derived progenitor cells (AHPCs) into dystrophic RCS rats). Surprisingly, AHPCs were also found to integrate successfully into a xenogeneic recipient (e.g., rat AHPCs into the retina of dystropic rd-I mice).

    摘要翻译： 本发明涉及修复成熟动物特别是神经元组织如视网膜的受损或患病，专门或分化组织的方法。 特别地，本发明涉及将成年的海马衍生的祖细胞移植到接受者的所选择的神经组织部位。 这些细胞可以功能整合到成熟和未成熟的神经组织中。 本发明在一个方面包括通过将衍生自健康供体动物的克隆衍生的成体中枢神经系统干细胞（ACSC）注射到营养不良的接受者的眼睛中，将营养不良的动物的视网膜与神经元重新填充。 本文公开了克隆衍生的ACSC在相同物种但不同的菌株接收者（例如，Fischer大鼠衍生的成年海马衍生的祖细胞（AHPC））到营养不良的RCS大鼠中的第一次成功和稳定的整合。 令人惊讶的是，还发现AHPC成功地融合到异种受体（例如，大鼠AHPC进入dystropic rd-I小鼠的视网膜）中。