A bottle for containing pharmacological and/or nutritional active substances is described. The bottle comprises a closing cap pierceable by the pressure exerted with the luer of a needle-free syringe. The closing cap is made as a single piece including a check valve and closing lips which can be opened by introducing a syringe luer and can be elastically closed back upon the extraction of the luer introduced, and a membrane which is pierceable and elastically resealable to hermetically close the inlet end of the check valve.