A chassis structure capable of sensing temperature of a media storage device includes a tray. The tray includes a body, an accommodating space formed in the body, a connector disposed on the body, and a temperature sensor juxtaposed with the connector. The media storage device is electrically coupled to the connector and contacts the temperature sensor. A working temperature is measured by a temperature sensor directly attached to an outer housing of the media storage device, thereby avoiding influences such as air flow and undesired heat conduction of the chassis structure.