To provide a multilayer structure having high gas bather property and crack resistance as well as excellent fatigue resistance, an inner liner for a pneumatic tire using the multilayer structure, and a pneumatic tire comprising the inner liner. According to the present invention, the multilayer structure includes a barrier layer 2 made of polymer material with 50% or less elongation at break conforming to JIS K 7113 at 20° C. and 65% RH and an elastomer layer 3 made of polymer material with 100% or more elongation at break conforming to JIS K 7113 at 20° C. and 65% RH, wherein a plastic deformation amount at the time of 100% strain input is larger than a deformation amount at a tolerance point.