The present disclosure provides a method for rubbing an alignment layer on a substrate with a plurality of spacers that are arranged in rows and columns and a liquid crystal display panel. The method includes: determining a first rubbing direction and a second rubbing direction in such a manner that the second rubbing direction is an arrangement direction of liquid crystal molecules when the liquid crystal molecules are arranged correctly on the alignment layer, and an angle between the first rubbing direction and the second rubbing direction is greater than or equal to arctan (b/a), where a represents a row pitch between the spacers and b represents a width of one spacer; performing a first rubbing on the alignment layer in the first rubbing direction; and performing a second rubbing on the alignment layer in the second rubbing direction. The second rubbing direction is different from the first rubbing direction.