A display panel for 3D display is disclosed. It includes a plurality of pixel units arranged in rows and columns, each of the plurality of pixel units including a plurality of sub pixels. The pixel units in adjacent columns are used as left-eye pixel units and right-eye pixel units, respectively, and intermediate sub pixels are provided between the sub pixels of the pixel units in the adjacent columns, and brightness values output by the intermediate sub pixels are equal to zero. A display device and a pixel driving method are also disclosed. The display panel, the display device and the pixel driving method can reduce a crosstalk caused by the light leakage of the grating, thereby improving a display performance of the display device.