- 专利标题: Vectors with modified initiation codon for the translation of AAV-REP78 useful for production of AAV
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申请号: US15424560申请日: 2017-02-03
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公开(公告)号: US09988645B2公开(公告)日: 2018-06-05
- 发明人: Wilhelmus Theodorus Johannes Maria Christiaan Hermens , Saskia Jacoba Petronella Haast , Dennis Johan Biesmans , Andrew Christian Bakker
- 申请人: uniQure IP B.V.
- 申请人地址: NL Amsterdam
- 专利权人: uniQure IP B.V.
- 当前专利权人: uniQure IP B.V.
- 当前专利权人地址: NL Amsterdam
- 代理机构: Foley & Lardner LLP
- 代理商 Gilberto M. Villacorta; Sunit Talapatra
- 优先权: EP06115804 20060621
- 主分类号: C12N7/00
- IPC分类号: C12N7/00 ; C12N15/86 ; C07K14/005 ; C12N15/864 ; C12N5/10
摘要:
The present invention relates nucleic acid constructs for the production of recombinant parvoviral (e.g. adeno-associated viral) vectors in insect cells, to insect cells comprising such constructs and to methods wherein the cells are used to produce recombinant parvoviral virions. The insect cells preferably comprise a first nucleotide sequence encoding the parvoviral rep proteins whereby the initiation codon for translation of the parvoviral Rep78 protein is a suboptimal initiation codon that effects partial exon skipping upon expression in insect cells. The insect cell further comprises a second nucleotide sequence comprising at least one parvoviral (AA V) inverted terminal repeat (ITR) nucleotide sequence and a third nucleotide sequence comprising a sequences coding for the parvoviral capsid proteins.
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