再颁专利
- 专利标题: Detection of nucleic acid amplification
- 专利标题(中): 检测核酸扩增
-
申请号: US09082247申请日: 1998-05-20
-
公开(公告)号: USRE39885E1公开(公告)日: 2007-10-16
- 发明人: James G. Nadeau , George T. Walker
- 申请人: James G. Nadeau , George T. Walker
- 申请人地址: US NJ Franklin Lakes
- 专利权人: Becton, Dickinson and Company
- 当前专利权人: Becton, Dickinson and Company
- 当前专利权人地址: US NJ Franklin Lakes
- 代理商 Allan M. Kiang
- 主分类号: C12Q1/68
- IPC分类号: C12Q1/68 ; C12P19/34
摘要:
Methods for detecting, immobilizing or localizing primer extension products of a Strand Displacement Amplification reaction which are coupled to, and an indication of, amplification of the target sequence. The primer extension products are secondary, target-specific DNA products generated concurrently with SDA of the target sequence and can therefore be used to detect and/or measure target sequence amplification in real-time. In general, the secondary amplification products are not amplifiable and remain inert in the SDA reaction after they are formed without interfering with amplification of the target sequence. The secondary amplification products may be designed or modified to contain special features to facilitate their detection, immobilization or localization.