Invention Application
WO2004074498A3 EXPRESSION OF CLASS 2 MANNOSIDASE AND CLASS III MANNOSIDASE IN LOWER EUKARYOTIC CELLS
审中-公开
在较低的原核细胞中表达2类曼卡塞菌素和第3类芒果苷
- Patent Title: EXPRESSION OF CLASS 2 MANNOSIDASE AND CLASS III MANNOSIDASE IN LOWER EUKARYOTIC CELLS
- Patent Title (中): 在较低的原核细胞中表达2类曼卡塞菌素和第3类芒果苷
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Application No.: PCT/US2004005132Application Date: 2004-02-20
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Publication No.: WO2004074498A3Publication Date: 2005-06-23
- Inventor: HAMILTON STEPHEN R , GERNGROSS TILLMAN U , WILDT STEFAN , CHOI BYUNG-KWON , NETT JUERGEN HERMANN , BOBROWICZ PIOTR , DAVIDSON ROBERT C
- Applicant: HAMILTON STEPHEN R , GERNGROSS TILLMAN U , WILDT STEFAN , CHOI BYUNG-KWON , NETT JUERGEN HERMANN , BOBROWICZ PIOTR , DAVIDSON ROBERT C
- Assignee: HAMILTON STEPHEN R,GERNGROSS TILLMAN U,WILDT STEFAN,CHOI BYUNG-KWON,NETT JUERGEN HERMANN,BOBROWICZ PIOTR,DAVIDSON ROBERT C
- Current Assignee: HAMILTON STEPHEN R,GERNGROSS TILLMAN U,WILDT STEFAN,CHOI BYUNG-KWON,NETT JUERGEN HERMANN,BOBROWICZ PIOTR,DAVIDSON ROBERT C
- Priority: US37187703 2003-02-20; US61608203 2003-07-08
- Main IPC: C12N9/10
- IPC: C12N9/10 ; C12N15/79 ; C12P21/00 ; C12N5/10 ; C12N9/24 ; C12N15/81 ; C12R1/645
Abstract:
A method for producing human-like glycoproteins by expressing a Class 2 alpha-mannosidase having a substrate specificity for Manalphal,3 and Manalphal,6 glycosidic linkages in a lower eukaryote is disclosed. Hydrolysis of these linkages on oligosaccharides produces substrates for further N-glycan processing in the secretory pathway.
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