公开(公告)号：WO2023060308A1

公开(公告)日：2023-04-20

申请号：PCT/AU2022/051227

申请日：2022-10-12

Applicant: MONASH UNIVERSITY

Inventor： DALEY, Stephen Robert ,  GRAS, Stephanie ,  LA GRUTA, Nicole ,  ZAREIE, Pirooz ,  SZETO, Christopher

IPC: C12N15/79 ,  C12N5/0783 ,  C07K14/005 ,  C07K14/47 ,  C07K14/725 ,  A61K35/17 ,  A61K38/00

Abstract: The present invention relates to modified T cell receptors and their uses in treating various diseases or conditions, particularly cancer and autoimmune diseases. A binding protein comprising a variable domain comprising a complementarity-determining region (CDR) capable of contacting a peptide bound to an HLA molecule, wherein the CDR 5 comprises a cysteine capable of forming a disulphide bond with a cysteine in the peptide bound to the HLA molecule, typically wherein the cysteine is introduced into the CDR by mutation or modification of an existing residue.

公开(公告)号：WO2023015222A1

公开(公告)日：2023-02-09

申请号：PCT/US2022/074492

申请日：2022-08-03

Applicant: BAYLOR COLLEGE OF MEDICINE

Inventor： ROSENGART, Todd ,  PINNAMANENI, Jayapratap ,  SINGH, Vivek P. ,  YANG, Jiangchang

IPC: C07K14/47 ,  A61K38/00 ,  C12N15/79 ,  A61P35/00 ,  A61P9/00

Abstract: Embodiments of the disclosure include methods and compositions for in situ cardiac cell regeneration, including transdifferentiation of cardiac cells to cardiomyocytes. In particular embodiments, in situ cardiac cell regeneration encompasses delivery of p63-TID and one or both of Hand2 and myocardin, and in specific embodiments further includes one or more of Gata4, Mef2c, and Tbx5, and/or one or more of ETV2 and VEGF. In specific aspects of the disclosure, adult cardiac fibroblasts are reprogrammed into cardiomyocytes using viral vectors that harbor p63-TID and one or both of the transcription factors Hand2 and myocardin.

公开(公告)号：WO2023008664A1

公开(公告)日：2023-02-02

申请号：PCT/KR2021/018446

申请日：2021-12-07

Applicant: 그린미네랄 주식회사

Inventor： 정광환 ,  심진곤 ,  이호석

IPC: C12N15/79 ,  C07K14/405 ,  C12N15/87

Abstract: 본 발명은 클로렐라 불가리스 (Chlorella vulgaris) 형질전환용 벡터 시스템, 이를 이용한 클로렐라 불가리스 형질전환 방법 및 클로렐라 불가리스 형질전환체에 관한 것이다.

公开(公告)号：WO2022261490A2

公开(公告)日：2022-12-15

申请号：PCT/US2022/033091

申请日：2022-06-10

Applicant: ORNA THERAPEUTICS, INC.

Inventor： WESSELHOEFT, Robert Alexander ,  OTT, Kristen ,  BARNES, Thomas ,  MOTZ, Gregory ,  BECKER, Amy M. ,  HORHOTA, Allen T. ,  GOODMAN, Brian ,  SHU, Huan ,  SHIVASHANKAR, Varun

IPC: C12N15/79 ,  C12N15/85

Abstract: Circular RNA, along with related compositions and methods are described herein. In some embodiments, the inventive circular RNA comprises group I intron fragments, spacers, an IRES, duplex forming regions, and an expression sequence. In some embodiments, the expression sequence encodes an antigen. In some embodiments, circular RNA of the invention has improved expression, functional stability, immunogenicity, ease of manufacturing, and/or half-life when compared to linear RNA. In some embodiments, inventive methods and constructs result in improved circularization efficiency, splicing efficiency, and/or purity when compared to existing RNA circularization approaches.

公开(公告)号：WO2022247943A1

公开(公告)日：2022-12-01

申请号：PCT/CN2022/095749

申请日：2022-05-27

Applicant: SHANGHAI CIRCODE BIOMED CO., LTD.

Inventor： YANG, Yun ,  WANG, Zefeng

IPC: C12N15/67 ,  A61K48/00 ,  C12N15/79

Abstract: The present invention relates to a construct and method for preparing a circular RNA, and the use of the circular RNA. In particular, the present invention relates to a construct and method for preparing a circular RNA on the basis of a group II intron, and the use of the circular RNA.

公开(公告)号：WO2022246276A2

公开(公告)日：2022-11-24

申请号：PCT/US2022/030371

申请日：2022-05-20

Applicant: ALLEN INSTITUTE

Inventor： DAIGLE, Tanya ,  LEIN, Edward Sebastian ,  LEVI, Boaz P. ,  MICH, John K. ,  MITCHELL, Amanda ,  TASIC, Bosiljka ,  TING, Jonathan ,  WEED, Natalie ,  ZENG, Hongkui

IPC: C12N15/79 ,  C12N15/86 ,  G01N33/543

Abstract: Artificial expression constructs for modulating gene expression in targeted central nervous system cell types are described. The artificial expression constructs can be used to express synthetic genes or modify gene expression in GABAergic or glutamatergic neurons within the thalamus. In some cases, the artificial constructs can also be used to express synthetic genes or modify gene expression in neurons within the thalamus as well as a secondary cell type.

公开(公告)号：WO2022229854A1

公开(公告)日：2022-11-03

申请号：PCT/IB2022/053881

申请日：2022-04-27

Applicant: NOVARTIS AG

Inventor： BREES, Dominique ,  FREYSE, Sebastian ,  KAMMÜLLER, Michael ,  LAUX, Holger ,  RAHN, Beat Christoph ,  WOLF, Babette

IPC: C12N9/10 ,  C12N15/79

Abstract: The present invention pertains to mammalian cells with increased sialylation activity. The mammalian cells are transfected with coding sequences of a sialyltransferase, a galactosyltransferase and a sialic acid transporter, resulting in hypersialylation of recombinantly expressed glycoproteins.

公开(公告)号：WO2022214705A1

公开(公告)日：2022-10-13

申请号：PCT/EP2022/059587

申请日：2022-04-11

Applicant: CILIAN AG

Inventor： HARTMANN, Marcus Wolf William ,  ALDAG, Ingo ,  ROSSDORF, Jan ,  UTHOFF, Stefan ,  SCHUMACHER, Florian

IPC: C07K14/44 ,  C12N9/20 ,  C12N9/28 ,  C12N9/50 ,  C12N15/79 ,  C12N9/2417

Abstract: The present invention relates to a method for purifying proteins, which proteins are produced by homologous or heterologous expression in a ciliate host, from harvested cell culture fluid (HCCF) which was not chromatographically purified. The method comprises the steps of incubating a harvested cell culture fluid with a kosmotropic agent, efflorescing protein by formation of crystals, and, optionally, harvesting effloresced protein.

公开(公告)号：WO2022132520A1

公开(公告)日：2022-06-23

申请号：PCT/US2021/062321

申请日：2021-12-08

Applicant: PIONEER HI-BRED INTERNATIONAL, INC.

Inventor： DAVIS-VOGEL, Courtney Anne ,  MENDEZ, Edwin J. ,  SAMUEL, Jayakumar Pon ,  THILGES, Katherine A.

IPC: C07K7/00 ,  C12N15/87 ,  C12N15/79 ,  C12N15/113 ,  A01N25/00

Abstract: A method of introducing a molecule of interest into an insect or an insect cell includes interacting a cell-penetrating peptide with a molecule of interest to form a complex. The structure is then placed in contact with an insect or an insect cell and allowing uptake of the complex into the insect or insect cell. Alternatively, an RNA polynucleotide (mRNA, or an RNAi-mediating molecule) can be introduced in an insect or an insect cell by interacting a peptide with the polynucleotide of interest to form a complex, allowing uptake of the RNA complex into the insect or insect cell, and expressing the polynucleotide in the insect or an insect cell.

公开(公告)号：WO2022115506A2

公开(公告)日：2022-06-02

申请号：PCT/US2021/060685

申请日：2021-11-24

Applicant: UNIVERSITY OF HOUSTON SYSTEM

Inventor： VARADARAJAN, Navin ,  SADEGHI, Fatemeh

IPC: C12N15/79

Abstract: The present disclosure is directed to composition and methods that enable salicylic acid-inducible gene expression in host cells.