Invention Application
- Patent Title: METHOD FOR THE ISOLATION OF RNA FROM BIOLOGICAL SOURCES
- Patent Title (中): 从生物资源中分离RNA的方法
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Application No.: PCT/US2006/026618Application Date: 2006-07-07
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Publication No.: WO2007008722A2Publication Date: 2007-01-18
- Inventor: CHEN, Fuqiang , CUTTER, David
- Applicant: SIGMA-ALDRICH CO. , CHEN, Fuqiang , CUTTER, David
- Applicant Address: 3050 Spruce Street, St. Louis, Missouri 63103 US
- Assignee: SIGMA-ALDRICH CO.,CHEN, Fuqiang,CUTTER, David
- Current Assignee: SIGMA-ALDRICH CO.,CHEN, Fuqiang,CUTTER, David
- Current Assignee Address: 3050 Spruce Street, St. Louis, Missouri 63103 US
- Agency: MCBRIDE, Timothy, B. et al.
- Priority: US11/180,087 20050713
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C07H21/02
Abstract:
Methods and kits for isolating RNA are provided that enable rapid RNA preparation from biological sources. In one aspect, RNA is isolated from difficult plant tissues and cells that contain high levels of secondary metabolites, without employing organic extraction or salt precipitation procedures. This method employs novel lysing and binding conditions to allow preparation of RNA free from secondary metabolites.
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