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1.发明申请A UNIVERSAL LIGATION ARRAY FOR ANALYZING GENE EXPRESSION OR GENOMIC VARIATIONS 审中-公开用于分析基因表达或基因变异的通用阵列
公开(公告)号：WO2009029742A1
公开(公告)日：2009-03-05
申请号：PCT/US2008/074716
申请日：2008-08-29
Applicant: SIGMA-ALDRICH CO. , CHEN, Fuqiang
Inventor： CHEN, Fuqiang
CPC classification number: C12Q1/6809 , C12Q2565/519 , C12Q2561/125 , C12Q2525/207
Abstract: The present invention provides an array system comprising a plurality of immobilized oligonucleotides comprising artificial sequences and a plurality of complementary ligation templates, as well as methods and kits for using the array system to analyze populations of nucleic acids. In particular, target nucleic acids are ligated to the immobilized oligonucleotides on the array in the presence of the complementary ligation templates.
Abstract translation: 本发明提供了一种阵列系统，其包括多个固定寡核苷酸，其包含人工序列和多个互补连接模板，以及使用阵列系统分析核酸群体的方法和试剂盒。 特别地，在互补连接模板存在下，将目标核酸连接到阵列上的固定化寡核苷酸。
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公开(公告)号：WO2007008722A3
公开(公告)日：2007-03-01
申请号：PCT/US2006026618
申请日：2006-07-07
Applicant: SIGMA ALDRICH CO , CHEN FUQIANG , CUTTER DAVID
Inventor： CHEN FUQIANG , CUTTER DAVID
IPC: C07H21/00
CPC classification number: C12Q1/6806 , C12Q2527/125 , C12Q2523/308 , C12Q2523/113
Abstract: Methods and kits for isolating RNA are provided that enable rapid RNA preparation from biological sources. In one aspect, RNA is isolated from difficult plant tissues and cells that contain high levels of secondary metabolites, without employing organic extraction or salt precipitation procedures. This method employs novel lysing and binding conditions to allow preparation of RNA free from secondary metabolites.
Abstract translation: 提供用于分离RNA的方法和试剂盒，其能够从生物来源快速制备RNA。 在一个方面，RNA从含有高水平次级代谢物的困难的植物组织和细胞中分离，而不使用有机提取或盐沉淀方法。 该方法采用新的裂解和结合条件以制备不含次级代谢物的RNA。
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公开(公告)号：WO2005111059A2
公开(公告)日：2005-11-24
申请号：PCT/US2005/013376
申请日：2005-04-20
Applicant: SIGMA-ALDRICH CO. , RAY, Kevin B. , KREADER, Carol A. , CHEN, Fuqiang , CUTTER, David E.
Inventor： RAY, Kevin B. , KREADER, Carol A. , CHEN, Fuqiang , CUTTER, David E.
IPC: C07H21/04
CPC classification number: C07H21/04 , C12N15/101
Abstract: The present invention provides methods for the reduction of endotoxins in a plasmid preparation using a carbohydrate non-ionic detergent with silica chromatography.
Abstract translation: 本发明提供了使用碳水化合物非离子型去垢剂与二氧化硅色谱法在质粒制备物中还原内毒素的方法。
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4.发明申请GENOME EDITING USING TARGETING ENDONUCLEASES AND SINGLE-STRANDED NUCLEIC ACIDS 审中-公开使用靶向内切酶和单条核酸的基因组编辑
公开(公告)号：WO2012012738A1
公开(公告)日：2012-01-26
申请号：PCT/US2011/045037
申请日：2011-07-22
Applicant: SIGMA-ALDRICH CO., LLC , CHEN, Fuqiang , PRUETT-MILLER, Shondra, M. , DAVIS, Gregory, D.
Inventor： CHEN, Fuqiang , PRUETT-MILLER, Shondra, M. , DAVIS, Gregory, D.
CPC classification number: C12N15/85 , C12N9/22 , C12N15/907
Abstract: The present invention provides methods and kits for editing specific chromosomal sequences in cells. In particular, targeting endonucleases and single-stranded nucleic acids are used to edit the chromosomal sequence.
Abstract translation: 本发明提供用于编辑细胞中特定染色体序列的方法和试剂盒。 特别地，靶向核酸内切酶和单链核酸用于编辑染色体序列。
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公开(公告)号：WO2008097342A2
公开(公告)日：2008-08-14
申请号：PCT/US2007074751
申请日：2007-07-30
Applicant: SIGMA ALDRICH CO , CHEN FUQIANG , KREADER CAROL
Inventor： CHEN FUQIANG , KREADER CAROL
IPC: B01D39/00
CPC classification number: C12N15/1003
Abstract: The present invention provides charged separation media, kits comprising the separation media, and methods of using the same to bind and optionally isolate biological molecules. The charged separation media and methods utilize a polyion non-covalently bound to the separation media to bind the desired biological molecule to the separation media.
Abstract translation: 本发明提供带电分离介质，包含分离介质的试剂盒，以及使用它们结合并任选分离生物分子的方法。 带电分离介质和方法利用与分离介质非共价结合的聚离子将所需生物分子结合到分离介质。
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Patent Agency Ranking
公开(公告)号：WO2005068662A1
公开(公告)日：2005-07-28
申请号：PCT/US2004/043980
申请日：2004-12-30
Applicant: SIGMA-ALDRICH CO. , CHEN, Fuqiang
Inventor： CHEN, Fuqiang
IPC: C12Q1/68
CPC classification number: C12N15/1003 , C12Q1/6806
Abstract: The present invention provides methods, compositions and kits for isolating and purifying at least one nucleic acid directly from a biological sample, or for preparing a biological sample for subsequent isolation and purification of at least one nucleic acid, without removal of the biological sample’s cell culture medium or cellular fluid.
Abstract translation: 本发明提供用于从生物样品直接分离和纯化至少一种核酸的方法，组合物和试剂盒，或用于制备用于随后分离和纯化至少一种核酸的生物样品，而不去除生物样品的细胞培养物 中等或细胞液体。
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公开(公告)号：WO2009043288A1
公开(公告)日：2009-04-09
申请号：PCT/CN2008/072515
申请日：2008-09-25
Applicant: CATERPILLAR INC. , MA, Jiubo , YANG, Zhishang , ZHAO, Shizhu , ZHANG, Shoudu , SHENG, Yuexuan , YANG, Lianmin , WANG, Zengqiang , CHEN, Fuqiang , XU, Xinqiang
Inventor： MA, Jiubo , YANG, Zhishang , ZHAO, Shizhu , ZHANG, Shoudu , SHENG, Yuexuan , YANG, Lianmin , WANG, Zengqiang , CHEN, Fuqiang , XU, Xinqiang
CPC classification number: F28F1/04 , B23K26/242 , B23K26/244 , F28D1/05366 , F28F2275/067
Abstract: A tube assembly (30) for a heat exchanger (10) is provided having a first U-shaped stainless steel assembly component (36) and a second U-shaped stainless steel assembly component (40). In addition, the tube assembly has at least one laser-welded joint coupling the first and second U-shaped assembly components together. The laser-welded joint has a weld that extends along substantially an entire length of the tube assembly.
Abstract translation: 提供了一种用于热交换器（10）的管组件（30），其具有第一U形不锈钢组件（36）和第二U形不锈钢组件部件（40）。 此外，管组件具有将第一和第二U形组件部件联接在一起的至少一个激光焊接接头。 激光焊接接头具有沿管组件的大致整个长度延伸的焊缝。
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公开(公告)号：WO2008097957A2
公开(公告)日：2008-08-14
申请号：PCT/US2008/053035
申请日：2008-02-05
Applicant: SIGMA-ALDRICH CO. , CHEN, Fuqiang
Inventor： CHEN, Fuqiang
CPC classification number: C12Q1/6816 , C12Q2561/125 , C12Q2525/207
Abstract: Methods, compositions, and kits for detecting mature small RNAs are provided herein. The methods comprise ligating at least one linker to a mature small RNA in the presence of a complementary ligation template to generate a ligation product. The ligation product is a hybrid molecule comprising the linker and the mature small RNA.
Abstract translation: 本文提供了用于检测成熟小RNA的方法，组合物和试剂盒。 所述方法包括在互补连接模板存在下将至少一个接头连接至成熟小RNA以产生连接产物。 连接产物是包含接头和成熟小RNA的杂合分子。
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公开(公告)号：WO2007008722A2
公开(公告)日：2007-01-18
申请号：PCT/US2006/026618
申请日：2006-07-07
Applicant: SIGMA-ALDRICH CO. , CHEN, Fuqiang , CUTTER, David
Inventor： CHEN, Fuqiang , CUTTER, David
CPC classification number: C12Q1/6806 , C12Q2527/125 , C12Q2523/308 , C12Q2523/113
Abstract: Methods and kits for isolating RNA are provided that enable rapid RNA preparation from biological sources. In one aspect, RNA is isolated from difficult plant tissues and cells that contain high levels of secondary metabolites, without employing organic extraction or salt precipitation procedures. This method employs novel lysing and binding conditions to allow preparation of RNA free from secondary metabolites.
Abstract translation: 提供了用于分离RNA的方法和试剂盒，其能够从生物来源快速制备RNA。 在一个方面，RNA是从困难的植物组织和含有高水平次级代谢物的细胞中分离出来的，而不采用有机提取或盐沉淀程序。 这种方法采用新的裂解和结合条件来制备不含次生代谢物的RNA。
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公开(公告)号：WO2008097957A3
公开(公告)日：2008-12-04
申请号：PCT/US2008053035
申请日：2008-02-05
Applicant: SIGMA ALDRICH CO , CHEN FUQIANG
Inventor： CHEN FUQIANG
IPC: C12Q1/68
CPC classification number: C12Q1/6816 , C12Q2561/125 , C12Q2525/207
Abstract: Methods, compositions, and kits for detecting mature small RNAs are provided herein. The methods comprise ligating at least one linker to a mature small RNA in the presence of a complementary ligation template to generate a ligation product. The ligation product is a hybrid molecule comprising the linker and the mature small RNA.
Abstract translation: 本文提供了用于检测成熟小RNA的方法，组合物和试剂盒。 该方法包括在互补连接模板存在下将至少一个接头连接至成熟小RNA以产生连接产物。 连接产物是包含接头和成熟小RNA的杂交分子。
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