Invention Application
- Patent Title: HYBRIDIZATION METHODS USING NATURAL BASE MISMATCHES
- Patent Title (中): 使用自然基因杂交的杂交方法
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Application No.: PCT/CN2007/000402Application Date: 2007-02-06
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Publication No.: WO2007090345A1Publication Date: 2007-08-16
- Inventor: GAO, Huafang , LI, Ze , WANG, Dong , LIU, Yanhua , LIU, Xiang , JIANG, Yangzhou , LI, Li , ZHAO, Chuanzan , LAN, Gengxin , GUO, Tao , CAI, Bin , CHENG, Jing
- Applicant: CAPITALBIO CORPORATION , TSINGHUA UNIVERSITY , GAO, Huafang , LI, Ze , WANG, Dong , LIU, Yanhua , LIU, Xiang , JIANG, Yangzhou , LI, Li , ZHAO, Chuanzan , LAN, Gengxin , GUO, Tao , CAI, Bin , CHENG, Jing
- Applicant Address: 18 Life Science Parkway, Changping, Beijing 102206 CN
- Assignee: CAPITALBIO CORPORATION,TSINGHUA UNIVERSITY,GAO, Huafang,LI, Ze,WANG, Dong,LIU, Yanhua,LIU, Xiang,JIANG, Yangzhou,LI, Li,ZHAO, Chuanzan,LAN, Gengxin,GUO, Tao,CAI, Bin,CHENG, Jing
- Current Assignee: CAPITALBIO CORPORATION,TSINGHUA UNIVERSITY,GAO, Huafang,LI, Ze,WANG, Dong,LIU, Yanhua,LIU, Xiang,JIANG, Yangzhou,LI, Li,ZHAO, Chuanzan,LAN, Gengxin,GUO, Tao,CAI, Bin,CHENG, Jing
- Current Assignee Address: 18 Life Science Parkway, Changping, Beijing 102206 CN
- Agency: JEEKAI & PARTNERS
- Priority: CN200610002863.8 20060208
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C12N15/00
Abstract:
The present invention provides an improved nucleic acid hybridization process employing a modified oligonucleotide probe comprising naturally occurring nucleotide bases. At least one nucleotide in the modified oligonucleotide is artificially mismatched relative to the control nucleic acid in addition to any mismatches arising from a variant nucleic acid target containing a sequence variation. The artificial mismatch and the sequence variation positions are separated from one another on the oligonucleotide by six to nine nucleotide positions.
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