公开(公告)号：WO2007090345A1

公开(公告)日：2007-08-16

申请号：PCT/CN2007/000402

申请日：2007-02-06

Applicant: CAPITALBIO CORPORATION ,  TSINGHUA UNIVERSITY ,  GAO, Huafang ,  LI, Ze ,  WANG, Dong ,  LIU, Yanhua ,  LIU, Xiang ,  JIANG, Yangzhou ,  LI, Li ,  ZHAO, Chuanzan ,  LAN, Gengxin ,  GUO, Tao ,  CAI, Bin ,  CHENG, Jing

Inventor： GAO, Huafang ,  LI, Ze ,  WANG, Dong ,  LIU, Yanhua ,  LIU, Xiang ,  JIANG, Yangzhou ,  LI, Li ,  ZHAO, Chuanzan ,  LAN, Gengxin ,  GUO, Tao ,  CAI, Bin ,  CHENG, Jing

IPC: C12Q1/68 ,  C12N15/00

CPC classification number: C12Q1/6881 ,  C12Q1/6832 ,  C12Q2600/156 ,  C12Q2535/131 ,  C12Q2527/107

Abstract: The present invention provides an improved nucleic acid hybridization process employing a modified oligonucleotide probe comprising naturally occurring nucleotide bases. At least one nucleotide in the modified oligonucleotide is artificially mismatched relative to the control nucleic acid in addition to any mismatches arising from a variant nucleic acid target containing a sequence variation. The artificial mismatch and the sequence variation positions are separated from one another on the oligonucleotide by six to nine nucleotide positions.

Abstract translation: 本发明提供使用包含天然存在的核苷酸碱基的修饰的寡核苷酸探针的改进的核酸杂交方法。 除了由含有序列变异的变体核酸靶产生的任何错配之外，修饰寡核苷酸中至少一个核苷酸相对于对照核酸人为错配。 在寡核苷酸上将人工错配和序列变异位置彼此分开6至9个核苷酸位置。

公开(公告)号：WO2007056904A1

公开(公告)日：2007-05-24

申请号：PCT/CN2006/000237

申请日：2006-02-20

Applicant: CAPITALBIO CORPORATION ,  TSINGHUA UNIVESITY ,  GAO, Huafang ,  LI, Ze ,  WANG, Dong ,  LIU, Yanhua ,  LIU, Xiang ,  JIANG, Yangzhou ,  ZHAO, Chuanzan ,  LI, Li ,  LAN, Gengxin ,  GUO, Tao ,  CAI, Bin ,  XING, Wanli ,  ZHOU, Yuxiang ,  CHENG, Jing

Inventor： GAO, Huafang ,  LI, Ze ,  WANG, Dong ,  LIU, Yanhua ,  LIU, Xiang ,  JIANG, Yangzhou ,  ZHAO, Chuanzan ,  LI, Li ,  LAN, Gengxin ,  GUO, Tao ,  CAI, Bin ,  XING, Wanli ,  ZHOU, Yuxiang ,  CHENG, Jing

IPC: C12Q1/68

CPC classification number: C12Q1/6881 ,  C12Q1/6837 ,  C12Q2600/156

Abstract: The present invention provides a microarray for detecting a genotype at a polymorphic site in a plurality of nucleic acid samples, comprising a first set of nucleic acid fragments derived from the samples and a second set of nucleic acid fragments derived from a plurality of references immobilized thereon. The invention also provides a microarray comprising a set of nucleic acid fragments immobilized on the surface of the microarray, wherein the nucleic acid fragments are derived from the samples by amplifying a region in the sample containing the polymorphism through asymmetric PCR amplification. Methods of using and making the microarrays are also provided.

Abstract translation: 本发明提供了用于检测多个核酸样品中多态性位点处的基因型的微阵列，其包含衍生自样品的第一组核酸片段和衍生自多个固定在其上的参考文献的第二组核酸片段 。 本发明还提供了一种微阵列，其包含固定在微阵列表面上的一组核酸片段，其中通过不对称PCR扩增扩增含有多态性的样品中的区域，来从样品衍生出核酸片段。 还提供了使用和制造微阵列的方法。

公开(公告)号：WO2005001123A1

公开(公告)日：2005-01-06

申请号：PCT/CN2003/000580

申请日：2003-07-18

Applicant: TSINGHUA UNIVERSITY ,  CAPITAL BIOCHIP COMPANY, LTD. ,  GAO, Huafang ,  MA, Xuemei ,  ZHANG, Chi ,  CHEN, Qian ,  ZHOU, Yiming ,  WANG, Dong ,  ZHANG, Yizhe ,  TIAN, Yue ,  ZHANG, Rui ,  LAN, Gengxin ,  ZHOU, Yuxiang ,  CHENG, Jing

Inventor： GAO, Huafang ,  MA, Xuemei ,  ZHANG, Chi ,  CHEN, Qian ,  ZHOU, Yiming ,  WANG, Dong ,  ZHANG, Yizhe ,  TIAN, Yue ,  ZHANG, Rui ,  LAN, Gengxin ,  ZHOU, Yuxiang ,  CHENG, Jing

IPC: C12Q1/68

CPC classification number: C12Q1/6837

Abstract: This invention relates generally to the field of nucleic acid analysis. In particular, the invention provides a method for typing a target gene, using, inter alia, a chip comprising a support suitable for use in nucleic acid hybridization having immobilized thereon an oligonucleotide probe complementary to said target nucleotide sequence and at least one of the following oligonucleotide control probes: a positive control probe, a negative control probe, a hybridization control probe and an immobilization control probe. Oligonucleotide probes or probes arrays for typing a HLA target gene are also provided.

Abstract translation: 本发明一般涉及核酸分析领域。 特别地，本发明提供了一种用于对目标基因进行分型的方法，特别地，使用包含适合用于核酸杂交的载体的芯片，其上固定有与所述靶核苷酸序列互补的寡核苷酸探针和至少一种以下 寡核苷酸对照探针：阳性对照探针，阴性对照探针，杂交对照探针和固定化对照探针。 还提供了用于分型HLA靶基因的寡核苷酸探针或探针阵列。