Invention Application
WO2008049781A1 METHOD OF REDUCING GENE EXPRESSION USING MODIFIED CODON USAGE
审中-公开
使用改性CODON使用减少基因表达的方法
- Patent Title: METHOD OF REDUCING GENE EXPRESSION USING MODIFIED CODON USAGE
- Patent Title (中): 使用改性CODON使用减少基因表达的方法
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Application No.: PCT/EP2007/061151Application Date: 2007-10-18
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Publication No.: WO2008049781A1Publication Date: 2008-05-02
- Inventor: HEROLD, Andrea , KLOPPROGGE, Corinna , SCHRÖDER, Hartwig , ZELDER, Oskar , JEONG, Weol Kyu
- Applicant: BASF SE , HEROLD, Andrea , KLOPPROGGE, Corinna , SCHRÖDER, Hartwig , ZELDER, Oskar , JEONG, Weol Kyu
- Applicant Address: 67056 Ludwigshafen DE
- Assignee: BASF SE,HEROLD, Andrea,KLOPPROGGE, Corinna,SCHRÖDER, Hartwig,ZELDER, Oskar,JEONG, Weol Kyu
- Current Assignee: BASF SE,HEROLD, Andrea,KLOPPROGGE, Corinna,SCHRÖDER, Hartwig,ZELDER, Oskar,JEONG, Weol Kyu
- Current Assignee Address: 67056 Ludwigshafen DE
- Agency: BUEHLER, Dirk
- Priority: EP06122882.1 20061024
- Main IPC: C12N15/77
- IPC: C12N15/77
Abstract:
The present invention is directed to a method of reducing the amount of at least one polypeptide in a host cell by expressing a nucleotide sequence encoding for the polypeptide in the host cell wherein the nucleotide sequence uses codons that are rarely used according to the codon usage of the host organism. Furthermore, the present invention relates to nucleotide sequences encoding for a polypeptide with a codon usage that has been adjusted to use codons that are only rarely used according to the codon usage of the host organism. The present invention further relates to the use of such sequences and methods for producing fine chemicals such as amino acids, sugars, lipids, oils, carbohydrates, vitamins, cofactors etc.
Information query
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