Invention Application
- Patent Title: METHOD FOR PURIFYING HUMAN GRANULOCYTE-COLONY STIMULATING FACTOR FROM RECOMBINANT E. COLI
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Application No.: PCT/KR2011/008046Application Date: 2011-10-26
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Publication No.: WO2012057529A3Publication Date: 2012-05-03
- Inventor: CHOI, Sung Chul , KIM, Jin Ki , OH, Young Hak , LEE, Jong Soo
- Applicant: HANMI SCIENCE CO., LTD. , CHOI, Sung Chul , KIM, Jin Ki , OH, Young Hak , LEE, Jong Soo
- Applicant Address: 550, Dongtangiheung-ro, Dongtan-myeon, Hwaseong-si, Gyeonggi-do 445-813 KR
- Assignee: HANMI SCIENCE CO., LTD.,CHOI, Sung Chul,KIM, Jin Ki,OH, Young Hak,LEE, Jong Soo
- Current Assignee: HANMI SCIENCE CO., LTD.,CHOI, Sung Chul,KIM, Jin Ki,OH, Young Hak,LEE, Jong Soo
- Current Assignee Address: 550, Dongtangiheung-ro, Dongtan-myeon, Hwaseong-si, Gyeonggi-do 445-813 KR
- Agency: SON, Min
- Priority: KR10-2010-0106994 20101029
- Main IPC: C07K14/53
- IPC: C07K14/53 ; C07K1/16 ; C12N15/27 ; C12N15/70
Abstract:
The present invention provides a method for purifying a large amount of human granulocyte-colony stimulating factors (hG-CSFs) from a recombinant E. coli with high yield and purity. According to the method of the present invention, human granulocyte-colony stimulating factor, identical to the native form expressed in the human body, can be easily purified with high yield and purity without an additional activation process. In particular, according to the purification method of the present invention, hG-CSF variants expressed in E. coli are efficiently removed to obtain physiologically active hG-CSFs with high purity.
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