Invention Application
- Patent Title: METHOD FOR DETECTING THE PRESENCE OF ONE OR MORE BACTERIAL TOXINS IN A BIOLOGICAL FLUID USING LIPOSOMES
-
Application No.: PCT/GB2018/051261Application Date: 2018-05-10
-
Publication No.: WO2018206961A1Publication Date: 2018-11-15
- Inventor: KADIOGLU, Aras , NEILL, Daniel , PANAGIOTOU, Stavros
- Applicant: THE UNIVERSITY OF LIVERPOOL
- Applicant Address: Foundation Building 765 Brownlow Hill Liverpool L69 7ZX GB
- Assignee: THE UNIVERSITY OF LIVERPOOL
- Current Assignee: THE UNIVERSITY OF LIVERPOOL
- Current Assignee Address: Foundation Building 765 Brownlow Hill Liverpool L69 7ZX GB
- Agency: HGF LIMITED
- Priority: GB1707559.9 20170511
- Main IPC: G01N33/50
- IPC: G01N33/50 ; G01N33/543 ; G01N33/569
Abstract:
The present invention relates to a method for detecting the presence of one or more bacterial toxins, capable of binding to cell membranes, in biological fluid wherein the method comprises: (i) incubating the biological fluid with a plurality of liposomes, wherein the liposomes comprise a lipid capable of binding to said one or more toxins, to provide one or more liposome-toxin conjugate; (ii) incubating said conjugates with at least one type of antibody bound to a label to provide one or more conjugate-antibody complex; wherein each type of antibody in the mixture is specific for one of the bacterial toxins whose presence is to be detected; and (iii) analysing said complexes in order to detect the presence of one or more bacterial toxins capable of binding to cell membranes. Further aspects of the invention relate to a conjugate-antibody complex useful in the methods of the invention and a kit useful for performing the method of the invention.
Information query
IPC分类: