公开(公告)号：WO2018206961A1

公开(公告)日：2018-11-15

申请号：PCT/GB2018/051261

申请日：2018-05-10

Applicant: THE UNIVERSITY OF LIVERPOOL

Inventor： KADIOGLU, Aras ,  NEILL, Daniel ,  PANAGIOTOU, Stavros

IPC: G01N33/50 ,  G01N33/543 ,  G01N33/569

Abstract: The present invention relates to a method for detecting the presence of one or more bacterial toxins, capable of binding to cell membranes, in biological fluid wherein the method comprises: (i) incubating the biological fluid with a plurality of liposomes, wherein the liposomes comprise a lipid capable of binding to said one or more toxins, to provide one or more liposome-toxin conjugate; (ii) incubating said conjugates with at least one type of antibody bound to a label to provide one or more conjugate-antibody complex; wherein each type of antibody in the mixture is specific for one of the bacterial toxins whose presence is to be detected; and (iii) analysing said complexes in order to detect the presence of one or more bacterial toxins capable of binding to cell membranes. Further aspects of the invention relate to a conjugate-antibody complex useful in the methods of the invention and a kit useful for performing the method of the invention.