- 专利标题： METHOD OF SIALYLATING A PROTEIN
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申请号： PCT/SG2020/050154申请日： 2020-03-23
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公开(公告)号： WO2020204814A1公开(公告)日： 2020-10-08
- 发明人: CHOO, Sern Farh Matthew , NGUYEN-KHUONG, Terry , PALLISTER, Edward George , NG, Say Kong , FLITSCH, Sabine Lahja
- 申请人： AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH , THE UNIVERSITY OF MANCHESTER
- 申请人地址： 1 Fusionopolis Way, #20-10 Connexis North Tower, Singapore 138632 SG
- 专利权人： AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH,THE UNIVERSITY OF MANCHESTER
- 当前专利权人： AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH,THE UNIVERSITY OF MANCHESTER
- 当前专利权人地址： 1 Fusionopolis Way, #20-10 Connexis North Tower, Singapore 138632 SG
- 代理机构： DAVIES COLLISON CAVE ASIA PTE. LTD.
- 优先权： SG10201902822V 20190329
- 主分类号： C12P21/00
- IPC分类号： C12P21/00 ; C07K14/81 ; A61K38/55 ; A61P11/00 ; A61P1/16
摘要：
The invention relates to a method of increasing the number of α2,3,-α2,6-disialylgalactose N -glycans on a glycoprotein by incubating an α2,3-sialylated glycoprotein with an α2,6-sialyltransferase and a sialic acid source. Also provided is a recombinant glycoprotein comprising at least one α2,3,-α2,6-disialylgalactose N -glycan. In a particular embodiment, the recombinant glycoprotein is alpha-1 antitrypsin (AAT).
