Provided are a method for enhancing the secretion function of mesenchymal stem cells and the use thereof. By means of using hyaluronic acid sodium (HA) to pretreat umbilical cord mesenchymal stem cells in vitro, the secretion function of the umbilical cord mesenchymal stem cells can be promoted, the secretion of factors such as HGF, SCF, EGF and VEGF is promoted, the activation of a PI3K-AKT signaling pathway is further promoted, and the repair effect thereof on injuries is enhanced. The method is simple, convenient, low in cost and high in safety, and has a good practical application value.