Disclosed herein are a micropipette and a biological sample preparation system comprising the micropipette. The micropipette comprises an outer cannula and a puncture tube. Upper and lower ends of the outer cannula are respectively provided with an opening; the puncture tube is arranged inside the outer cannula in a penetrating manner; the upper end of the puncture tube is provided with an opening; and the lower end of the puncture tube tapers into a conical tail end. The micropipette is operably connected to a working module of the biological sample preparation system, so as to execute a biological sample processing or detection step by utilizing the micropipette.