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公开(公告)号：EP0789843B1
公开(公告)日：2007-05-02
申请号：EP95906633.3
申请日：1994-09-06
摘要： An apparatus (10) for hematology testing has a sensing unit (20) defining a flow-through counting orifice (102) to analyze a blood sample, and a pump unit (16) having three syringes (28, 30, 32). First and second syringes (28, 30) are coupled to an inlet chamber (A) of the counting orifice (102) for simultaneously injecting a blood sample/reagent mixture and a sheath fluid respectively into the inlet chamber (A). A third syringe (32) is coupled to an outlet chamber (B) of the counting orifice (102) for removing fluid from the outlet chamber (B). A control unit (22) is coupled to the sensing unit (20) and a drive motor (72) for the syringes. The control unit (22) includes a database of reagent quantities corresponding to different animal species for the selection of appropriate reagent/blood mixtures in the analysis of blood from different animals.
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2.发明公开METHOD/APPARATUS FOR CELL DIFFERENTIATION MEASURING CELL SIZE, MEMBRANE INTEGRITY, INTRACELLULAR COMPLEXITY 失效方法/设备细胞分化用于测量ZELLENGRÖE，细胞膜的完整性和INTRAZELLULARENKOMPLEXITÄT。
公开(公告)号：EP1005641A1
公开(公告)日：2000-06-07
申请号：EP98920381.5
申请日：1998-05-12
IPC分类号： G01N27/00
CPC分类号： G01N15/1218
摘要： A method and apparatus are provided for differentiating cells in an automated particle analyzer (10), such as for use in hematological analysis, by measuring apparent cell size (or volume), membrane integrity and intracellular complexity ('voltaic fragility'). The cells are suspended in a selected reagent mixture. A predetermined dc voltage is applied across a sensing orifice (36) to thereby create a dc electric field within and around the orifice (36), and the reagent mixture is passed through the center of the orifice (36). The magnitude of the change in current flowing through dc electric field is measured therethrough, and based on the measured change, a signal is generated for each cell which is indicative of the apparent complexity of the cell. A visual display (54) is generated based on the signals facilitating differentiation of the cells.
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公开(公告)号：EP0789843A4
公开(公告)日：2000-12-27
申请号：EP95906633
申请日：1994-09-06
申请人： CDC TECHNOLOGIES INC
摘要： An apparatus (10) for hematology testing has a sensing unit (20) defining a flow-through counting orifice (102) to analyze a blood sample, and a pump unit (16) having three syringes (28, 30, 32). First and second syringes (28, 30) are coupled to an inlet chamber (A) of the counting orifice (102) for simultaneously injecting a blood sample/reagent mixture and a sheath fluid respectively into the inlet chamber (A). A third syringe (32) is coupled to an outlet chamber (B) of the counting orifice (102) for removing fluid from the outlet chamber (B). A control unit (22) is coupled to the sensing unit (20) and a drive motor (72) for the syringes. The control unit (22) includes a database of reagent quantities corresponding to different animal species for the selection of appropriate reagent/blood mixtures in the analysis of blood from different animals.
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公开(公告)号：EP0830609A4
公开(公告)日：1999-08-18
申请号：EP96920585
申请日：1996-05-31
申请人： CDC TECHNOLOGIES INC
CPC分类号： G01N35/1097 , G01N1/38 , G01N35/085 , Y10T436/117497
摘要： In an apparatus (10) for fluid analysis, such as hematologic analysis, a plurality of reagent-mixture components are each injected by a respective pump (P1...Pn) through a valve matrix (16) and into a flow-injection unit (24). The flow-injection unit (24) defines a mixing chamber (50) including a plurality of protuberances or nubs (54) projecting inwardly toward the center of the chamber, and spaced relative to each other both axially and radially. As the reagent-mixture components are injected into the mixing chamber (50), the nubs (54) agitate the fluid flow and create turbulence, thereby dispersing the reagent-mixture components and in turn mixing the components together to create a reagent mixture. The flow rates of the reagent-mixture components are adjusted in order to select the reagent-mixture ratio as the components are combined in the flow-injection unit (24) to thereby create the selected reagent mixture. Upon passage through the flow-injection unit (24), the reagent mixture is injected into a sensing unit (20) for analyzing a particle distribution of the mixture.
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公开(公告)号：EP0808434A1
公开(公告)日：1997-11-26
申请号：EP96906266.0
申请日：1996-02-06
IPC分类号： F16K11
CPC分类号： F16K11/022 , Y10T137/86879
摘要： In a valve assembly (10) exhibiting zero dead volume the port head (12, 112) is mounted on a solenoid (14) and defines an inlet port (16, 116) on one side of the valve seat (20, 120), and two outlet ports (18, 19, 118, 119) on the opposite side of the valve seat. A flexible diaphragm (22) is seated across the valve seat (20, 120) between the port head (12, 112) and the solenoid (14), and is sealed along its peripheral edge. A spring-biased valve rod (24) is coupled to the opposite side of the diaphragm (22) relative to the valve seat (20, 120), and the solenoid (14) is actuated to move the valve rod (24) and diaphragm (22) between open and closed positions. The valve assembly (10) is opened by actuating the solenoid (14) to drive the valve rod (24) and diaphragm (22) away from the valve seat (20, 120) and permit the passage of fluid across the valve seat between the inlet (16, 116) and outlet ports (18, 19, 118, 119).
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公开(公告)号：EP0789843A1
公开(公告)日：1997-08-20
申请号：EP95906633.0
申请日：1994-09-06
摘要： An apparatus (10) for hematology testing has a sensing unit (20) defining a flow-through counting orifice (102) to analyze a blood sample, and a pump unit (16) having three syringes (28, 30, 32). First and second syringes (28, 30) are coupled to an inlet chamber (A) of the counting orifice (102) for simultaneously injecting a blood sample/reagent mixture and a sheath fluid respectively into the inlet chamber (A). A third syringe (32) is coupled to an outlet chamber (B) of the counting orifice (102) for removing fluid from the outlet chamber (B). A control unit (22) is coupled to the sensing unit (20) and a drive motor (72) for the syringes. The control unit (22) includes a database of reagent quantities corresponding to different animal species for the selection of appropriate reagent/blood mixtures in the analysis of blood from different animals.
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7.发明公开METHOD/APPARATUS FOR CELL DIFFERENTIATION MEASURING CELL SIZE, MEMBRANE INTEGRITY, INTRACELLULAR COMPLEXITY 失效方法/设备细胞分化用于测量ZELLENGRÖE，细胞膜的完整性和INTRAZELLULARENKOMPLEXITÄT。
公开(公告)号：EP1005641A4
公开(公告)日：2002-08-07
申请号：EP98920381
申请日：1998-05-12
申请人： CDC TECHNOLOGIES INC
发明人： CARVER EDWARD L JR , DECAVA DAVID C
IPC分类号： G01N15/12 , G01N33/487 , G01N27/00
CPC分类号： G01N15/1218
摘要： A method and apparatus are provided for differentiating cells in an automated particle analyzer (10), such as for use in hematological analysis, by measuring apparent cell size (or volume), membrane integrity and intracellular complexity ("voltaic fragility"). The cells are suspended in a selected reagent mixture. A predetermined dc voltage is applied across a sensing orifice (36) to thereby create a dc electric field within and around the orifice (36), and the reagent mixture is passed through the center of the orifice (36). The magnitude of the change in current flowing through dc electric field is measured therethrough, and based on the measured change, a signal is generated for each cell which is indicative of the apparent complexity of the cell. A visual display (54) is generated based on the signals facilitating differentiation of the cells.
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公开(公告)号：EP0808434A4
公开(公告)日：1998-12-16
申请号：EP96906266
申请日：1996-02-06
申请人： CDC TECHNOLOGIES INC
CPC分类号： F16K11/022 , Y10T137/86879
摘要： In a valve assembly (10) exhibiting zero dead volume the port head (12, 112) is mounted on a solenoid (14) and defines an inlet port (16, 116) on one side of the valve seat (20, 120), and two outlet ports (18, 19, 118, 119) on the opposite side of the valve seat. A flexible diaphragm (22) is seated across the valve seat (20, 120) between the port head (12, 112) and the solenoid (14), and is sealed along its peripheral edge. A spring-biased valve rod (24) is coupled to the opposite side of the diaphragm (22) relative to the valve seat (20, 120), and the solenoid (14) is actuated to move the valve rod (24) and diaphragm (22) between open and closed positions. The valve assembly (10) is opened by actuating the solenoid (14) to drive the valve rod (24) and diaphragm (22) away from the valve seat (20, 120) and permit the passage of fluid across the valve seat between the inlet (16, 116) and outlet ports (18, 19, 118, 119).
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公开(公告)号：EP0830609A1
公开(公告)日：1998-03-25
申请号：EP96920585.0
申请日：1996-05-31
CPC分类号： G01N35/1097 , G01N1/38 , G01N35/085 , Y10T436/117497
摘要： In an apparatus (10) for fluid analysis, such as hematologic analysis, a plurality of reagent-mixture components are each injected by a respective pump (P1...Pn) through a valve matrix (16) and into a flow-injection unit (24). The flow-injection unit (24) defines a mixing chamber (50) including a plurality of protuberances or nubs (54) projecting inwardly toward the center of the chamber, and spaced relative to each other both axially and radially. As the reagent-mixture components are injected into the mixing chamber (50), the nubs (54) agitate the fluid flow and create turbulence, thereby dispersing the reagent-mixture components and in turn mixing the components together to create a reagent mixture. The flow rates of the reagent-mixture components are adjusted in order to select the reagent-mixture ratio as the components are combined in the flow-injection unit (24) to thereby create the selected reagent mixture. Upon passage through the flow-injection unit (24), the reagent mixture is injected into a sensing unit (20) for analyzing a particle distribution of the mixture.
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