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公开(公告)号:EP3081644A4
公开(公告)日:2017-05-24
申请号:EP14869024
申请日:2014-12-08
申请人: AMANO ENZYME INC
发明人: ISHIGAKI YUKI , ISHIHARA SATORU , TANAKA SHUN-ICHI
CPC分类号: C12N9/20 , A23C9/1216 , A23L27/25 , A23V2002/00 , C12Y301/01003
摘要: The present invention addresses a problem of providing a lipase derived from a microorganism that is specific for short-chain to medium-chain fatty acids. A modified lipase is obtained by making a substitution in the amino acid sequence of a Candida cylindracea derived lipase, wherein the substitution is (1) a substitution of asparagine for an amino acid corresponding to the amino acid at position 428 in the amino acid sequence set forth in SEQ ID NO: 1; or (2) a substitution of phenylalanine, methionine, or isoleucine for an amino acid corresponding to the amino acid at position 429 in the amino acid sequence set forth in SEQ ID NO: 1.
摘要翻译: 本发明涉及一种提供来源于短链中链链脂肪酸特异性微生物的脂肪酶的问题。 修饰的脂肪酶是通过在圆假丝酵母衍生的脂肪酶的氨基酸序列中进行取代而获得的,其中取代是(1)天冬酰胺取代对应于氨基酸序列组中428位氨基酸的氨基酸 在SEQ ID NO:1中; 或(2)苯丙氨酸,甲硫氨酸或异亮氨酸取代对应于SEQ ID NO:1所示氨基酸序列中第429位氨基酸的氨基酸。