-
公开(公告)号：EP3184633A4
公开(公告)日：2017-12-27
申请号：EP15834600
申请日：2015-08-13
申请人： AMANO ENZYME INC
CPC分类号： C12N9/2402 , C12N15/09 , C12P19/04 , C12P19/14 , C12Y302/01023
摘要： The present invention has a purpose of providing a technique for increasing the heat resistance of a ²-galactosidase. According to the present invention, in a reference ²-galactosidase amino acid sequence which shows a 90% or more identity to the amino acid sequence of SEQ ID NO: 4, proline is substituted for one or more amino acids selected from the group consisting of the following amino acids: (1) an amino acid corresponding to lysine at position 166 of the amino acid sequence of SEQ ID NO: 4, (2) an amino acid corresponding to glycine at position 307 of the amino acid sequence of SEQ ID NO: 4, and (3) an amino acid corresponding to alanine at position 833 of the amino acid sequence of SEQ ID NO: 4.
-
公开(公告)号：EP3081644A4
公开(公告)日：2017-05-24
申请号：EP14869024
申请日：2014-12-08
申请人： AMANO ENZYME INC
发明人： ISHIGAKI YUKI , ISHIHARA SATORU , TANAKA SHUN-ICHI
CPC分类号： C12N9/20 , A23C9/1216 , A23L27/25 , A23V2002/00 , C12Y301/01003
摘要： The present invention addresses a problem of providing a lipase derived from a microorganism that is specific for short-chain to medium-chain fatty acids. A modified lipase is obtained by making a substitution in the amino acid sequence of a Candida cylindracea derived lipase, wherein the substitution is (1) a substitution of asparagine for an amino acid corresponding to the amino acid at position 428 in the amino acid sequence set forth in SEQ ID NO: 1; or (2) a substitution of phenylalanine, methionine, or isoleucine for an amino acid corresponding to the amino acid at position 429 in the amino acid sequence set forth in SEQ ID NO: 1.
摘要翻译： 本发明涉及一种提供来源于短链中链链脂肪酸特异性微生物的脂肪酶的问题。 修饰的脂肪酶是通过在圆假丝酵母衍生的脂肪酶的氨基酸序列中进行取代而获得的，其中取代是（1）天冬酰胺取代对应于氨基酸序列组中428位氨基酸的氨基酸 在SEQ ID NO：1中; 或（2）苯丙氨酸，甲硫氨酸或异亮氨酸取代对应于SEQ ID NO：1所示氨基酸序列中第429位氨基酸的氨基酸。
-
公开(公告)号：EP3064581A4
公开(公告)日：2017-03-29
申请号：EP14850029
申请日：2014-09-26
申请人： AMANO ENZYME INC
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/38 , C12P19/04 , C12P19/12 , C12P19/14
CPC分类号： C12P19/14 , C12N9/2471 , C12P19/04 , C12P19/12 , C12Y302/01023
摘要： The usefulness of ² -galactosidases derived from Bacillus circulans is further enhanced so that they can be applied to new applications. The present invention provides a modified ² -galactosidase in which one or more amino acids selected from the group consisting of proline at position 182 (P182), tyrosine at position 187 (Y187), serine at position 188 (S188), tryptophan at position 405 (W405), alanine at position 406 (A406), glutamine at position 407 (Q407), tyrosine at position 449 (Y449), threonine at position 483 (T483), serine at position 512 (S512), serine at position 531 (S531), serine at position 533 (S533), serine at position 534 (S534), asparagine at position 550 (N550), glutamine at position 551 (Q551), tryptophan at position 593 (W593), tyrosine at position 598 (Y598), proline at position 602 (P602), proline at position 604 (P604), tyrosine at position 609 (Y609), lysine at position 612 (K612), and tyrosine at position 615 (Y615), or an amino acid(s) corresponding thereto, has/have been substituted by other amino acid in a ² -galactosidase consisting of the amino acid sequence of any of SEQ ID NOs. 1 to 4 or an amino acid sequence having 90% or more identity to the amino acid sequence set forth in any of SEQ ID NOs. 1 to 4.
摘要翻译： 衍生自环状芽孢杆菌的β-半乳糖苷酶的有用性进一步增强，从而可以将其应用于新的应用。 本发明提供了一种修饰的β-半乳糖苷酶，其中一个或多个氨基酸选自182位的脯氨酸（P182），187位的酪氨酸（Y187），第188位的丝氨酸（S188），405位的色氨酸 （W405），406位丙氨酸（A406），407位谷氨酰胺（Q407），449位酪氨酸（Y449），第483位苏氨酸（T483），512位丝氨酸（S512），531位丝氨酸 ），533位的丝氨酸（S533），534位的丝氨酸（S534），550位的天冬酰胺（N550），551位的谷氨酰胺（595），593位的色氨酸（W593），598位的酪氨酸（Y598） 位置602（P602）的脯氨酸，604位的脯氨酸（P604），609位的酪氨酸（Y609），612位的赖氨酸（K612）和615位的酪氨酸（Y615）或与之对应的氨基酸 在由SEQ ID NO中任一个的氨基酸序列组成的β-半乳糖苷酶中已被其它氨基酸取代。 1至4或与SEQ ID NO中任一个所示氨基酸序列具有90％或更高同一性的氨基酸序列。 1到4。
-
公开(公告)号：EP2687597A4
公开(公告)日：2014-12-10
申请号：EP12758032
申请日：2012-03-05
申请人： AMANO ENZYME INC
发明人： ISHIHARA SATORU
CPC分类号： C12N9/2408 , C12N9/1051 , C12N9/2428 , C12P19/04 , C12P19/16 , C12P19/18 , C12P19/20 , C12Y302/0102 , Y02P20/52
摘要： The object is to provide an ±-glucosidase in which a transglycosylation activity predominates, and use thereof, and the like. A modified ±-glucosidase consisting of an amino acid sequence in which one or two or more of the amino acid(s) selected from the group consisting of the following (1) to (14) has/have been substituted by other amino acid(s) in the amino acid sequence of ±-glucosidase: (1) an amino acid corresponding to the amino acid at the position 385 of the amino acid sequence of SEQ ID NO.1; (2) an amino acid corresponding to the amino acid at the position 491 of the amino acid sequence of SEQ ID NO.1; (3) an amino acid corresponding to the amino acid at the position 535 of the amino acid sequence of SEQ ID NO.1; (4) an amino acid corresponding to the amino acid at the position 450 of the amino acid sequence of SEQ ID NO.1; (5) an amino acid corresponding to the amino acid at the position 534 of the amino acid sequence of SEQ ID NO.1; (6) an amino acid corresponding to the amino acid at the position 537 of the amino acid sequence of SEQ ID NO.1; (7) an amino acid corresponding to the amino acid at the position 538 of the amino acid sequence of SEQ ID NO.1; (8) an amino acid corresponding to the amino acid at the position 554 of the amino acid sequence of SEQ ID NO.1; (9) an amino acid corresponding to the amino acid at the position 556 of the amino acid sequence of SEQ ID NO.1; (10) an amino acid corresponding to the amino acid at the position 579 of the amino acid sequence of SEQ ID NO. 2; (11) an amino acid corresponding to the amino acid at the position 585 of the amino acid sequence of SEQ ID NO. 2; (12) an amino acid corresponding to the amino acid at the position 630 of the amino acid sequence of SEQ ID NO. 1; (13) an amino acid corresponding to the amino acid at the position 683 of the amino acid sequence of SEQ ID NO.1; and (14) an amino acid corresponding to the amino acid at the position 689 of the amino acid sequence of SEQ ID NO.1.
-
-
-
搜索结果
4 条记录 (耗时 0.007 秒)
