公开(公告)号：EP3851853A1

公开(公告)日：2021-07-21

申请号：EP20152484.0

申请日：2020-01-17

申请人： Biotechnologicky Ustav AV CR, v.v.i. ,  PrimeCell Advanced Therapy, a.s. ,  Ceská zemedelská univerzita v Praze

发明人： Komrskova, Katerina ,  Postlerova, Pavla ,  Frolikova, Michaela ,  Zahradnicek, Michal ,  Pospisilova, Veronika ,  Forostyak, Serhiy ,  Simonik, Ondrej

IPC分类号： G01N33/50 ,  C12N5/076

摘要： The present invention provides a method of separation of sperm cells with undamaged intact heads from sperm cells with damaged heads and/or somatic cells and/or cell debris in a cell sample obtained from a human, comprising the steps of:
- providing an anti-CD46 antibody bound to a carrier and/or to a tag;
- contacting the cell sample with the anti-CD46 antibody bound to the carrier and/or to the tag in order to bind CD46 presenting cells and/or cell debris from the cell sample to the anti-CD46 antibody;
- removing the CD46 presenting cells bound via the anti-CD46 antibody to the carrier and/or to the tag to obtain a cell sample free of CD46 presenting cells, wherein CD46 presenting cells include sperm cells with damaged heads, somatic cells and cell debris.
The technology of the invention is in particular designed to separate sperm with undamaged intact heads to be further used in assisted reproduction, human medicine and research.

公开(公告)号：EP3851852A1

公开(公告)日：2021-07-21

申请号：EP20152470.9

申请日：2020-01-17

申请人： Biotechnologicky Ustav AV CR, v.v.i. ,  PrimeCell Advanced Therapy, a.s. ,  Ceská zemedelská univerzita v Praze

发明人： Komrskova, Katerina ,  Postlerova, Pavla ,  Frolikova, Michaela ,  Zahradnicek, Michal ,  Pospisilova, Veronika ,  Forostyak, Serhiy ,  Simonik, Ondrej

IPC分类号： G01N33/50 ,  G01N33/68

摘要： The present invention provides a method of separation of sperm cells with undamaged intact heads from sperm cells with damaged heads and/or somatic cells and/or cell debris in a cell sample obtained from an animal, comprising the steps of:
- providing an anti-CD46 antibody bound to a carrier and/or to a tag;
- contacting the cell sample with the anti-CD46 antibody bound to the carrier and/or to the tag in order to bind CD46 presenting cells and/or cell debris from the cell sample to the anti-CD46 antibody;
- removing the CD46 presenting cells bound via the anti-CD46 antibody to the carrier and/or to the tag to obtain a cell sample free of CD46 presenting cells, wherein CD46 presenting cells include sperm cells with damaged heads, somatic cells and cell debris.
This technology is designed to separate sperm with undamaged intact heads to be further used in assisted reproduction, medicine (in particular breeding programs and veterinary medicine), storage purpose, and research.